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http://krishi.icar.gov.in/jspui/handle/123456789/7059| Title: | Effect of lactate on Bovine Heart Mitochondria-Mediated Metmyoglobin Reduction |
| Other Titles: | Not Available |
| Authors: | Prince Devadason I Anjaneyulu ASR Babji Y |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Meat, Hyderabad |
| Published/ Complete Date: | 2010-12-31 |
| Project Code: | Not Available |
| Keywords: | Lactate Bovine Heart Mitochondria mediated metmyoglobin |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Our objective was to determine the combined effects of lactate, LDH, and NAD on metmyoglobin reduction in mitochondria isolated from bovine cardiac muscle. Mitochondria were reacted with various combinations of lactate, LDH, NAD, and mitochondrial inhibitors, and oxygen consumption was measured using a Clark oxygen electrode. Mitochondria (3 mg/mL) and bovine metmyoglobin (0.15 mM) also were reacted with substrates/enzymes/inhibitors to determine mitochondria-mediated metmyoglobin reduction in vitro. Combining lactate-LDH-NAD with isolated mitochondria increased oxygen consumption as well as metmyoglobin reduction compared with those of either control mitochondria (without lactate) or mitochondria with added lactate, at pH 5.6 and 7.4 (p < 0.05). The addition of mitochondrial and LDH inhibitors to lactate-LDH-NAD decreased oxygen consumption and metmyoglobin reduction (p < 0.05). NADH formed from lactate-LDH-NAD can be used for nonenzymatic (via the electron transport chain) and enzymatic (NADH-dependent metmyoglobin reductase) metmyoglobin |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Agricultural and Food Chemistry |
| NAAS Rating: | 10.19 |
| Volume No.: | 58 |
| Page Number: | 5724-5729 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://pubs.acs.org/doi/10.1021/jf1002842 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/7059 |
| Appears in Collections: | AS-NRCMeat-Publication |
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