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http://krishi.icar.gov.in/jspui/handle/123456789/71549
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Balamurugan V | en_US |
dc.contributor.author | Varghese B | en_US |
dc.contributor.author | SowjanyaKumari S | en_US |
dc.contributor.author | Vinod Kumar K | en_US |
dc.contributor.author | Muthuchelvan D | en_US |
dc.contributor.author | Nagalingam M | en_US |
dc.contributor.author | Hemadri D | en_US |
dc.contributor.author | Roy P | en_US |
dc.contributor.author | Shome BR | en_US |
dc.date.accessioned | 2022-04-18T09:07:46Z | - |
dc.date.available | 2022-04-18T09:07:46Z | - |
dc.date.issued | 2021-06-10 | - |
dc.identifier.citation | V. Balamurugan, Bibitha Varghese, S. SowjanyaKumari, K. Vinod Kumar, D. Muthuchelvan, M. Nagalingam, D. Hemadri, Parimal Roy, B.R. Shome, Avidin-Biotin recombinant nucleoprotein competitive ELISA for the detection of peste des petits ruminants virus antibodies in sheep and goats, Journal of Virological Methods, 295,(September): 114213 | en_US |
dc.identifier.issn | 0166-0934 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/71549 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The present study describes the development of a truncated recombinant peste des petits ruminants virus (PPRV) nucleoprotein (rPPRV-NPN) and its polyclonal antibodies-based immuno-diagnostic assay, Avidin-Biotin (AB) recombinant nucleoprotein competitive ELISA (ABrC-ELISA) for the detection of PPRV antibodies in the sheep and goats. The PPRV N-terminal immunogenic region (1–266 aa) of nucleoprotein (NPN) coding sequence was amplified and cloned into the pETite vector. The rPPRV-NPN with a molecular weight of ∼ 30 kDa was expressed in E. coli, purified, and characterized by SDS-PAGE and immunoblot using standard PPRV specific sera. The Ni-NTA affinity-purified rPPRV-NPN as coating antigen and its hyperimmune serum as competitive antibodies raised in guinea pigs were evaluated as diagnostic reagents in ABrC-ELISA using the known standard panel of sera. The threshold (cut-off) Percentage Inhibition (PI) value was determined as 45 (mean ± 3 SD) based on the reactivity of the known sheep and goats sera to PPRV antibodies [negative (n = 140) and positive (n = 98)] and the assay had a sensitivity of 97 % (95 % Confidence Interval (CI): 91.3–99.4 %) and specificity of 100 % (95 % CI: 97.4–100 %) with an excellent Area under curve (AUC) of 0.997 (95 % CI: 0.99–1.0). On evaluation of diagnostic performance of the assay using the sheep and goats sera (n = 391) from vaccinated, infected, and non-vaccinated animals, the ABrC-ELISA showed the relative diagnostic sensitivity of 95.88 % (95 % CI: 92.56–98.01 %) & 98.77 % (95 % CI: 96.43–99.74 %) and diagnostic specificity of 97.97 % (95 % CI: 94.19–99.58 %) & 90.54 % (95 % CI: 84.64–94.73 %) against indigenous PPR competitive ELISA kit & IDvet Screen® PPR Competition kit, respectively. The study showed that ABrC-ELISA is rapid, sensitive, and specific and can be a better alternative assay for the detection of the PPRV antibodies in the sera of small ruminants for serosurveillance / seromonitoring of PPR not only at the eradication and post-eradication phases in the disease-controlled endemic countries but also in the PPR non-endemic countries. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Elsevier Publisher | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | PPR | en_US |
dc.subject | Recombinant nucleoprotein | en_US |
dc.subject | Polyclonal antibodies | en_US |
dc.subject | Avidin-Biotin | en_US |
dc.subject | Competitive ELISA | en_US |
dc.subject | Surveillance | en_US |
dc.title | Avidin-Biotin recombinant nucleoprotein competitive ELISA for the detection of peste des petits ruminants virus antibodies in sheep and goats | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Virological Methods | en_US |
dc.publication.volumeno | 295 | en_US |
dc.publication.pagenumber | 1-10 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1016/j.jviromet.2021.114213 | en_US |
dc.publication.authorAffiliation | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics | en_US |
dc.publication.authorAffiliation | Division of Virology, ICAR-Indian Veterinary Research Institute, Campus Mukteswar-263 138, Nainital, Uttarakhand, India. | en_US |
dc.publication.authorAffiliation | Centre for Animal Health Studies, TANUVAS, Madhavaram Milk Colony, Chennai, 600 051, Tamil Nadu, India. | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Peer reviewed journal | en_US |
dc.publication.naasrating | 7.79 | en_US |
dc.publication.impactfactor | 2.014 | en_US |
Appears in Collections: | AS-NIVEDI-Publication |
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