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http://krishi.icar.gov.in/jspui/handle/123456789/71573
Title: | Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep. |
Other Titles: | Not Available |
Authors: | Mohanty NN Hemadri D Munivenkatarayappa A Shetty N Subramanyam V Biswas SK Chanda MM Shivachandra SB |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | National Institute of Veterinary Epidemiology and Disease Informatics ICAR-Indian Veterinary Research Institute, Mukteswar-Kumaon, Nainital 243122, Uttarakhand, India. |
Published/ Complete Date: | 2021-01-04 |
Project Code: | Not Available |
Keywords: | Bluetongue Diagnosis Indirect ELISA Non-structural protein Sero-surveillance |
Publisher: | Elsevier Publishing |
Citation: | Mohanty NN, Hemadri D, Munivenkatarayappa A, Shetty N, Subramanyam V, Biswas SK, Chanda MM, Shivachandra SB. Development of recombinant NS1-NS3 antigen based indirect ELISA for detection of bluetongue antibodies in sheep. J Immunol Methods. 2021 Mar;490:112959. doi: 10.1016/j.jim.2021.112959. Epub 2021 Jan 4. PMID: 33412173. |
Series/Report no.: | Not Available; |
Abstract/Description: | Bluetongue is an insect borne (Culicoides) viral disease of small ruminants. The virus blankets the globe with a wide serotypic variation, numbered from 1 to 28. In India 21 different serotypes have been reported to be circulating across the various agro-climatic zones of the country. Non-structural proteins (NSPs) of bluetongue virus have always remained ideal target for differentiation of infected from vaccinated animals. The current study is an extrapolation of our previous work where a novel fusion construct comprising of bluetongue viral segment NS1 and NS3 was successfully cloned, expressed, purified with an efficient strategy for its suitable implementation as a diagnostic antigen. In this study, the applicability of the fusion construct has been further evaluated and optimised for field applicability. The fusion construct used in an ELISA platform projected a relative diagnostic sensitivity and specificity of 98.1% and 95.5% respectively against a pre-established test panel. The rNS1-NS3 ELISA showed substantially good agreement with the commercial BTV antibody detection kit. Finally, the study brings together the diagnostic capability of two NSPs, which can be a handy tool for sero-surveillance of bluetongue. |
Description: | Not Available |
ISSN: | 0022-1759 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Immunological Methods |
Journal Type: | Peer reviewer journal |
NAAS Rating: | 7.9 |
Impact Factor: | 2.303 |
Volume No.: | 490 |
Page Number: | 1-6 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1016/j.jim.2021.112959 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/71573 |
Appears in Collections: | AS-NIVEDI-Publication |
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