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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/73973
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Not Available | en_US |
dc.date.accessioned | 2022-09-07T08:45:30Z | - |
dc.date.available | 2022-09-07T08:45:30Z | - |
dc.date.issued | 2013-01-25 | - |
dc.identifier.citation | Nair, R., Kalia, V., Aggarwal, K.K., and Gujar, G.T. 2013. Variation in the cadherin gene sequence of Cry1Ac susceptible and reistant Helicoverpa armigera (Lepidoptera:Noctuidae) and the identification of mutant alleles in resistant strains. Current Science. 104(2): 215-223. | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/73973 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Variations in the cadherin gene sequence between Cry1Ac-resistant BH-R strain showing 227.9-fold resistance and the wild susceptible VA-S strain of Helicoverpa armigera were identified. Amplification of exon 26 from H. armigera cadherin gene identified two mutant alleles, r9 (553 bp) and r10 (717 bp) against the wild S allele (588 bp). Sequence analysis of r9 showed a deletion of 45 bp in the intron region and mutation in the 5′-splice site of exon 26 resulted in an additional amino acid. While in the r10 allele a premature stop codon resulted in the putative translation into two different proteins of 1343 and 335 amino acids relative to a single protein of 1756 amino acids of the susceptible strain. The segregation of these mutant alleles was examined in F2 progenies derived from matings of Cry1Ac-resistant and susceptible individuals and found to be associated with Mendelian principles. Cadherin genotyping showed that resistance to Cry1Ac, survival on Cry1Ac-treated diet and frequency of mutant r allele of cadherin gene were high in the selected strain than the unselected strain and were completely absent from susceptible VA-S strain. DNA-based screening of H. armigera collected in India failed to detect a single r9 or r10 allele in the populations collected from vegetable-growing areas of Delhi. However, very low frequency of mutant alleles was detected in Bt cotton-growing areas of Anand, indicating that these mutations are likely to be rare in the field. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Current Science Association in collaboration with the Indian Academy of Sciences (India) | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Alleles, cadherin, Cry1Ac-resistance, Helicoverpa armigera, mutation | en_US |
dc.title | Variation in the cadherin gene sequence of Cry1Ac susceptible and reistant Helicoverpa armigera (Lepidoptera:Noctuidae) and the identification of mutant alleles in resistant strains. | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Current Science | en_US |
dc.publication.volumeno | 104 | en_US |
dc.publication.pagenumber | 215-223 | en_US |
dc.publication.divisionUnit | Entomology | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR::Indian Agricultural Research Institute | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | CS-IARI-Publication |
Files in This Item:
File | Description | Size | Format | |
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Current science -cadherin 2013.pdf | 972.26 kB | Adobe PDF | View/Open |
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