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http://krishi.icar.gov.in/jspui/handle/123456789/74061
Title: | A kidney bean trypsin inhibitor with an insecticidal potential against Helicoverpa armigera and Spodoptera litura. |
Other Titles: | Not Available |
Authors: | Mittal, A, Kansal, R, Kalia, V., Tripathi, M. & Gupta, V.K. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Agricultural Research Institute |
Published/ Complete Date: | 2013-11-15 |
Project Code: | Not Available |
Keywords: | Kidney bean Trypsin inhibitor Helicoverpa armigera Spodoptera litura Artificial diet Insect bioassay |
Publisher: | Springer |
Citation: | Mittal, A, Kansal, R, Kalia, V. Tripathi, M. & Gupta, V.K. 2013. A kidney bean trypsin inhibitor with an insecticidal potential against Helicoverpa armigera and Spodoptera litura. Acta Physiologiae Plantarum.36(2): 525-539. http//DOI 10.1007/s11738-013-1433-4.Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | In the present study, trypsin inhibitor extracts of ten kidney bean seed (Phaseolus vulgaris) varieties exhibiting trypsin and gut trypsin-like protease inhibitor activity were tested on Helicoverpa armigera and Spodoptera litura. Trypsin inhibitor protein was isolated and purified using multi-step strategy with a recovery of *15 % and purification fold by *39.4. SDS-PAGE revealed a single band corresponding to molecular mass of *15 kDa and inhibitory activity was confirmed by reverse zymogram analyses. The inhibitor retained its inhibitory activity over a broad range of pH (3–11), temperature (40–60 C) and thermostability was promoted by casein, CaCl2, BSA and sucrose. The purified inhibitor inhibited bovine trypsin in 1:1 molar ratio. Kinetic studies showed that the protein is a competitive inhibitor with an equilibrium dissociation constant of 1.85 lM. The purified trypsin inhibitor protein was further incorporated in the artificial diet and fed to second instar larvae. A maximum of 91.7 % inhibition was obtained in H. armigera, while it was moderate in S. litura (29 %) with slight varietal differences. The insect bioassay showed 40 and 22 % decrease in larval growth followed by 3 and 2 days delay in pupation of H. armigera and S. litura, respectively. Some of the adults emerged were deformed and not fully formed. Trypsin inhibitor protein was more effective against H. armigera as it showed 46.7 % mortality during larval growth period compared to S. litura (13.3 %). |
Description: | Not Available |
ISSN: | 0137-5881 |
Type(s) of content: | Book Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Acta Physiologiae Plantarum |
Volume No.: | 36 |
Page Number: | 525-539 |
Name of the Division/Regional Station: | Entomology |
Source, DOI or any other URL: | DOI 10.1007/s11738-013-1433-4 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/74061 |
Appears in Collections: | CS-IARI-Publication |
Files in This Item:
File | Description | Size | Format | |
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acta_physiologea_plantarum.pdf | 1.27 MB | Adobe PDF | View/Open |
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