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http://krishi.icar.gov.in/jspui/handle/123456789/75266| Title: | Development of competitive allele specific PCR based SNP genotyping assays for breeding applications in castor |
| Other Titles: | Not Available |
| Authors: | Manmode Darpan Mohanrao M. Sujatha S. Vanisri B. Vidya Sagar Y. Rushwanth Reddy K. Divya Sravanthi S. Senthilvel |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Institute of Oilseeds Research Professor Jayashankar Telangana State Agricultural University |
| Published/ Complete Date: | 2022-07-01 |
| Project Code: | IIOR103-14 |
| Keywords: | Ricinus communis KASP assay Molecular markers Linkage map |
| Publisher: | Indian Society of Genetics & Plant Breeding |
| Citation: | Mohanrao MD, Sujatha M, Vanisri S, Sagar BV, Reddy YR, Sravanthi KD, Senthilvel S. 2022. Development of competitive allele specific PCR based SNP genotyping assays for breeding applications in castor. Indian Journal of Genetics and Plant Breeding 82(3): 350-354. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Castor is an industrially important oilseed crop, which provides raw material to many industries. Despite its industrial importance, the genomic resources available for molecular breeding applications are limited. In the present study, a set of 135 SNP loci polymorphic between two castor inbred lines namely, JC-12 and RG-1963 were targeted to develop competitive allele specific PCR assays for genotyping purposes. The assays were validated in the F2 population of JC-12 × RG-1963 cross. Out of 135 assays designed, 124 were found to be successful in discriminating three expected genotypes in the F2 population. Using the SNP genotyping data, a genetic linkage map representing 10 haploid chromosomes of castor was constructed, which corresponded very well with the physical map. This set of validated SNP markers is a useful resource for application in molecular breeding of castor. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | ICAR |
| Language: | English |
| Name of Journal: | Indian Journal of Genetics and Plant Breeding |
| Journal Type: | Included NAAS journal list |
| NAAS Rating: | 6.51 |
| Impact Factor: | 0.51 |
| Volume No.: | 82(3) |
| Page Number: | 350-354 |
| Name of the Division/Regional Station: | Crop Improvement |
| Source, DOI or any other URL: | doi: 10.31742/ISGPB.82.3.10 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/75266 |
| Appears in Collections: | CS-IIOR-Publication |
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