Identification of simple sequence repeat markers linked to Fusarium wilt (Fusarium oxysporum f.sp. carthami) resistance and marker assisted selection for wilt resistance in safflower (Carthamus tinctorius L.) interspecific offsprings.

Abstract

Safflower (Carthamus tinctorius L.) is a multipurpose oilseed crop. Fusarium wilt (Fusarium oxysporum f.sp. carthami) is the major damaging disease in safflower. In the present investigation, Fusarium wilt resistance was introgressed from two wild species, Carthamus oxyacantha and Carthamus palaestinus, into susceptible cultivated species through interspecific hybridization. Inheritance of wilt resistance indicated single dominant gene control. Eight simple‐sequence‐repeat (SSR) markers each in (‘Nira’ × C. oxyacantha) and (‘Nira’ × C. palaestinus) were found to be linked to wiltresistance. Marker‐assisted selection for wilt resistance was performed using these markers in F3–F7 generations of both crosses. Six wilt resistant interspecific lines evaluated for 2 years under nondisease conditions have recorded 9%–29% higher seed yield than the high yielding cultivar, ‘A1’. The wilt resistant lines would serve as new sources of resistance to wilt in safflower. The SSR markers linked to wilt resistance would be useful for precise selection of wilt resistance at seedling stage in large segregating populations without attempting screening in artificially inoculated conditions and pyramiding of wilt resistant genes from wild into a common background.