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http://krishi.icar.gov.in/jspui/handle/123456789/76663
Title: | Proteomic analysis of differential expression of lung proteins in response to highly pathogenic avian influenza virus infection in chickens |
Other Titles: | Not Available |
Authors: | Vijayakumar, P. Raut, A. A. Chingtham, S, Murugkar, H. V. Kulkarni, D. D. Sood, R. Singh, V. P. Mishra, A. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute of High Security Animal Diseases Veterinary College and Research Institute, Tamil Nadu Veterinary and Animal Sciences University, Orathanadu, 614625, Tamil Nadu, India. |
Published/ Complete Date: | 2022-01-01 |
Project Code: | Not Available |
Keywords: | H5N1 HPAI virus infection in chickens, Proteomic analysis |
Publisher: | Wien, New York, Springer-Verlag. |
Citation: | Vijayakumar, P., Raut, A. A., Chingtham, S., Murugkar, H. V., Kulkarni, D. D., Sood, R., Singh, V. P., & Mishra, A. (2022). Proteomic analysis of differential expression of lung proteins in response to highly pathogenic avian influenza virus infection in chickens. Archives of virology, 167(1) |
Series/Report no.: | Not Available; |
Abstract/Description: | Elucidation of the molecular pathogenesis underlying virus-host interactions is important for the development of new diagnostic and therapeutic strategies against highly pathogenic avian influenza (HPAI) virus infection in chickens. However, the pathogenesis of HPAI virus in chickens is not completely understood. To identify the intracellular signaling pathways and critical host proteins associated with influenza pathogenesis, we analyzed the lung proteome of a chicken infected with HPAI H5N1 virus (A/duck/India/02CA10/2011/Agartala). Mass spectrometry data sets were searched against the chicken UniProt reference database. At the local false discovery rate level of 5%, a total of 3313 proteins with the presence of at least one unique peptide were identified in the chicken lung proteome datasets. Differential expression analysis of these proteins showed that 247 and 1754 proteins were downregulated at 12 h and 48 h postinfection, respectively. We observed expression of proteins of the predominant signaling pathways, including Toll-like receptors (TLRs), retinoic acid-inducible gene I-like receptors (RLRs), NOD-like receptors (NLRs), and JAK-STAT signaling. Activation of these pathways is associated with the cytokine storm effect and thus may be the cause of the severity of HPAI H5N1 infection in chickens. We also observed the expression of myeloid differentiation primary response protein (MyD88), inhibitor of nuclear factor kappa B kinase subunit beta (IKBKB), interleukin 1 receptor associated kinase 4 (IRAK4), RELA proto-oncogene NF-κB subunit (RELA), and mitochondrial antiviral signaling protein (MAVS), which are involved in critical signaling pathways, as well as other, less-commonly identified proteins such as hepatocyte nuclear factor 4 alpha (HNF4A), ELAV-like RNA binding protein 1 (ELAVL1), fibronectin 1 (FN1), COP9 signalosome subunit 5 (COPS5), cullin 1 (CUL1), breast cancer type 1 susceptibility protein (BRCA1), and the FYN proto-oncogene Src family tyrosine kinase (FYN) as main hub proteins that might play important roles in influenza pathogenesis in chickens. In summary, we identified the signaling pathways and the proteomic determinants associated with disease pathogenesis in chickens infected with HPAI H5N1 virus. |
Description: | Not Available |
ISSN: | 0304-8608 |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Archives of virology |
Journal Type: | Included in NAAS journal list |
NAAS Rating: | 8.69 |
Impact Factor: | 2.69 |
Volume No.: | 167(1) |
Page Number: | 141–152 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1007/s00705-021-05287-5 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/76663 |
Appears in Collections: | AS-NIHSAD-Publication |
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