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http://krishi.icar.gov.in/jspui/handle/123456789/76849| Title: | Development of recombinant partial nucleoprotein of rabies virus as the potential antigen for developing in house immunodiagnostics |
| Other Titles: | Not Available |
| Authors: | Varughese HS Chandrahasa C Kavitha GM Akhila DS Gopinath S Govindaiah K Chandan T Rao VCP Hosamani M Ramakrishnaiah S Doddamane R Hemadri D Isloor S |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Department of Veterinary Microbiology, Veterinary College, KVAFSU, Bengaluru, Karnataka, India Nitte University Centre for Science Education and Research, Deralakatte, Mangaluru, Karnataka, India ICAR Indian Veterinary Research Institute, Bengaluru, Karnataka, India ICAR National Institute of Veterinary Epidemiology and Disease Informatics, Bengaluru, Karnataka, India |
| Published/ Complete Date: | 2022-12-12 |
| Project Code: | Not Available |
| Keywords: | Rabies nucleoprotein cloning in house immunodiagnostics |
| Publisher: | The Pharma Innovation |
| Citation: | Varughese HS, Chandrahasa C, Kavitha GM, Akhila DS, Gopinath S, Govindaiah K, Chandan T, Rao VCP, Hosamani M, Ramakrishnaiah S, Doddamane R, Hemadri D and Isloor S. (2022). Development of recombinant partial nucleoprotein of rabies virus as the potential antigen for developing in house immunodiagnostics. The Pharma Innovation Journal, 12 516-519 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Rabies viral nucleoprotein is proven to be antigenic and immunogenic in nature. Monoclonal antibody binding characterises the Rabies viral nucleoproteins immune reactivity. However, studies on reactivity of nucleoproteins to polyclonal antibodies are limited. In this study, a partial Rabies viral nucleoprotein gene of 516 bp was cloned into a pET 32a vector, and prokaryotic expression was induced to obtain a protein of 24.1 kDa. The protein obtained was further characterised by Western blot and indirect ELISA. Our research discovered Rabies nucleoprotein immune reactivity in polyclonal sera and suspected that the epitopes are conformational in nature. This study adds to the limited number of polyclonal sera based studies on Rabies nucleoprotein. |
| Description: | Not Available |
| ISSN: | 2277-7695 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | The Pharma Innovation Journal |
| Journal Type: | Peer reviewed journal |
| NAAS Rating: | 5.23 |
| Impact Factor: | Not listed in Impact Factor List |
| Volume No.: | 12 |
| Page Number: | 516-519 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://www.thepharmajournal.com/archives/2023/vol12issue1/PartF/11-12-644-103.pdf |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/76849 |
| Appears in Collections: | AS-NIVEDI-Publication |
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