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http://krishi.icar.gov.in/jspui/handle/123456789/77059| Title: | Rescue of infective virus from a genome- length cDNA clone of the FMDV serotype O (IND-R2/75) vaccine strain and its characterization. |
| Other Titles: | Not Available |
| Authors: | R Rajasekhar M Hosamani S H Basagoudanavar B P Sreenivasa R P Tamil Selvan P Saravanan R Venkataramanan |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2013-08-01 |
| Project Code: | Not Available |
| Keywords: | foot and mouth disease virus infectious cDNA Viral genome |
| Publisher: | Research in Veterinary Science |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | We report here the construction and characterization of an infectious cDNA clone of the Indian vaccine strain of foot and mouth disease virus (FMDV) serotype O, IND-R2/75. Viral genome was amplified by reverse transcription-polymerase chain reaction (RT-PCR) in five fragments and subsequently assembled sequentially in a plasmid vector to generate a complete cDNA clone, flanked by the T7 RNA polymerase promoter and poly (A) tail at 5′ and 3′ ends, respectively. Transfection of BHK-21 cells with the RNA transcribed from this genome-length cDNA construct allowed the recovery of infectious recombinant FMDV particles as evidenced by cytopathic effect in BHK-21 cells. Characterization of the recombinant virus revealed its similarity to the parental strain. Recombinant virus could be distinguished from the parental virus based on the presence of a unique marker sequence in the former, which was incorporated in the cDNA using a silent mutation. The virus showed no significant amino acid changes in the capsid-coding region when serially passaged up to ten times in BHK-21 cells, while retaining the marker sequence. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Research in Veterinary Science |
| Journal Type: | Included NAAS journal list |
| NAAS Rating: | 8.55 |
| Impact Factor: | 2.55 |
| Volume No.: | 95(1) |
| Page Number: | 291-7 |
| Name of the Division/Regional Station: | ICAR::Indian Veterinary Research Institute bengaluru |
| Source, DOI or any other URL: | https://doi.org/10.1016/j.rvsc.2013.01.026 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/77059 |
| Appears in Collections: | AS-IVRI-Publication |
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