KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/80791| Title: | ISOLATION AND MOLECULAR IDENTIFICATION OF DAMPING-OFF DISEASE CAUSATIVE PATHOGEN ( PYTHIUM SP.) IN TOBACCO NURSERIES USING ITS SEQUENCE CHARACTERIZATION |
| Other Titles: | Not Available |
| Authors: | K. PRABHAKARA RAO K. SARALA T.G.K. MURTHY S. K. DAM M. SUMA K. SIVARAJU M. SHESHU MADHAV |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Central Tobacco Research Institute |
| Published/ Complete Date: | 2022-06-30 |
| Project Code: | Not Available |
| Keywords: | ITS, Phythium Damping off Nursery and Pathogen |
| Publisher: | Indian Society of Tobacco Science |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Damping-off disease is caused by several species of fungi found under various weather conditions in nurseries throughout the world. It is a serious problem in tobacco nurseries, both in heavy clay and light sandy soils. Different classes of fungi viz. Rhizoctonia Fusarium, Pythium etc. can cause and have similar symptoms of Damping-off disease in various crops. In order to identify the damping-off disease causative agent at molecular level, samples were collected from damping-off infected tobacco nursery fields of Central Tobacco Research Institute (CTRI), Rajahmundry and its Research stations viz., Jeelugumilli, Dinhata and Hunsur. A total of 16 pathogen isolates were collected from the infected soils and cultured on PDA medium. Genomic DNA was isolated from all these pathogen isolates and purified. Intact genomic DNA of all the isolates were analysed with ITS (Internally Transcribed Sequence) specific primers using polymerse chain reaction. The PCR amplicons of ITS region comprising ITS1, ITS2 and 5.8S regions of all the isolates were purified and sequenced. The sequences obtained were annotated using NCBI BLAST and compared with the Gen Bank submitted sequences. The results revealed that Pythium spp are the predominant causative agent of damping-off disease in tobacco nurseries and P. aphanideramtum. is the most prevalent species. These findings will aid in designing the appropriate control measures against the specific pathogen (Pythium) to protect the tobacco nurseries from damping-off disease. |
| Description: | Not Available |
| ISSN: | 0379-055X |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Tobacco Research |
| Journal Type: | Not Available |
| NAAS Rating: | 3.33 |
| Impact Factor: | Not Available |
| Volume No.: | 48(2) |
| Page Number: | 66-70 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/80791 |
| Appears in Collections: | CS-CTRI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| TR 48(2) 66-70.pdf | 429.71 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.