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http://krishi.icar.gov.in/jspui/handle/123456789/81610
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sowjayna Kumari S | en_US |
dc.contributor.author | Bokade PP | en_US |
dc.contributor.author | Kumar KV | en_US |
dc.contributor.author | Bharath V | en_US |
dc.contributor.author | Shome BR | en_US |
dc.contributor.author | Balamurugan V | en_US |
dc.date.accessioned | 2024-03-06T15:50:34Z | - |
dc.date.available | 2024-03-06T15:50:34Z | - |
dc.date.issued | 2023-03-31 | - |
dc.identifier.citation | Sowjanya Kumari, S., Bokade, P.P., Kumar, K.V., Bharath, V., Shome, B., Balamurugan, V. (2023). Potential diagnostic application of the baculovirus-expressed recombinant truncated nucleocapsid protein of peste des petits ruminants virus in ELISA. Journal of Immunological Methods, 516: 113469. | en_US |
dc.identifier.issn | 0022-1759 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/81610 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The study describes the expression of recombinant truncated nucleocapsid protein (NP) of peste des petits ruminants (PPR) virus in the baculovirus system (PPRV-rBNP) and its potential application as a diagnostic antigen in ELISA for diagnosis of PPR in sheep and goats. The PPRV N-terminal immunogenic region (1–266 aa) of the NP coding sequence was amplified and cloned into the pFastBac HT A vector. The PPRV-rBNP with a molecular weight of ∼30 kDa was expressed in an insect cell system using generated recombinant baculovirus through Bac-to-Bac® Baculovirus Expression System. The crude PPRV-rBNP or Ni-NTA affinity-purified NP was characterized by SDS-PAGE and immunoblot using standard PPRV-specific sera. The PPRV-rBNP reacted well with PPRV anti-N specific monoclonal and polyclonal antibodies and PPRV-specific antiserum, suggesting that the expressed PPRV-rBNP is in its native form. The crude PPRV-rBNP as a diagnostic antigen was evaluated either as a coating antigen or standard positive control antigen in the Avidin-Biotin ELISA using the known standard panel reagents. The results showed that the expressed PPRV-rBNP can be an alternative diagnostic antigen to E. coli expressed recombinant PPRV-NPN and the utility of PPRV-rBNP avoids the need to use live PPRV antigen in the diagnostic ELISA. Hence, this allows scope in the future for large-scale field application of the recombinant antigen-based assays for diagnosis/surveillance and monitoring of PPR at the eradication as well as post-eradication phases in endemic countries or PPR non-endemic countries. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Elsevier Publishers | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Diagnostics | en_US |
dc.subject | PPR | en_US |
dc.subject | ELISA | en_US |
dc.title | Potential diagnostic application of the baculovirus-expressed recombinant truncated nucleocapsid protein of peste des petits ruminants virus in ELISA | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Journal of Immunological Methods | en_US |
dc.publication.volumeno | 516 | en_US |
dc.publication.pagenumber | 113469 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1016/j.jim.2023.113469 | en_US |
dc.publication.authorAffiliation | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics | en_US |
dc.publication.authorAffiliation | Jain University, Bengaluru, Karnataka, India | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.journaltype | Included NAAS Journal List | en_US |
dc.publication.naasrating | 8.20 | en_US |
dc.publication.impactfactor | 2.20 | en_US |
Appears in Collections: | AS-NIVEDI-Publication |
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