KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/81989| Title: | Development of multiplex PCR assay for simultaneous detection of African swine fever, porcine circo and porcine parvo viral infection from clinical samples |
| Other Titles: | Not Available |
| Authors: | Pegu, S.R., Deb, R., Das, P.J., Sengar, G.S., Yadav, A.K., Rajkhowa, S., Paul, S. and Gupta, V.K. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Pig |
| Published/ Complete Date: | 2022-03-28 |
| Project Code: | Not Available |
| Keywords: | Multiplex, PCV, PPV, ASF |
| Publisher: | Not Available |
| Citation: | Pegu, S.R., Deb, R., Das, P.J., Sengar, G.S., Yadav, A.K., Rajkhowa, S., Paul, S. and Gupta, V.K., 2023. Development of multiplex PCR assay for simultaneous detection of African swine fever, porcine circo and porcine parvo viral infection from clinical samples. Animal Biotechnology, 34(6), pp.1883-1890. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A diagnostic method for simultaneously detecting and distinguishing African Swine Fever (ASF), porcine circovirus type 2 (PCV2), and porcine parvovirus (PPV) in clinical specimens is critical for differential diagnosis, monitoring, and control in the field. Three primer pairs were designed and used to create a multiplex PCR assay. In addition, 356 porcine post mortem tissue samples from various parts of India's North Eastern region were tested by the developed multiplex PCR assay to demonstrate its accuracy. Using the designed primers, each of the ASF, PCV2 and PPV target genes was amplified, but no other porcine virus genes were detected. The assay's limit of detection was 102 copies/µl of PCV2, PPV, or ASFV. The detection of PCV2, PPV, and ASF in postmortem tissue samples revealed that they are co-circulating in India's North-Eastern region. The percentage positivity (PP) for PCV2, PPV and ASF single infection were 7.02% (25/356), 3.93% (14/356), and 3.37% (12/356), respectively, while the PP for PCV2& PPV co-infection was 2.80% (10/356), ASF & PCV2 co infection was 1.4% (5/356) and the ASF, PPV& PCV2 co-infection was1.40% (5/356). The results also indicate that the ASF can infect pigs alongside PCV and PPV. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Animal Biotechnology |
| Journal Type: | NAAS listed |
| NAAS Rating: | 9.70 |
| Impact Factor: | 3.7 |
| Volume No.: | 34(6) |
| Page Number: | 1883-1890 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | https://doi.org/10.1080/10495398.2022.2053698 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/81989 |
| Appears in Collections: | AS-NRCP-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.