Transcriptome analysis and identification of leaf, tuberous root and fibrous root tissue-specific high temperature stress-responsive genes in sweet potato

Abstract

Sweet Potato is an important food crop, and its production is affected by environmental stresses, including high temperature. The gene expression patterns and molecular responses in different tissues of sweet potato under high temperature stress were studied using microarray data sets. Analysis revealed that modulation in the expression of key genes and pathways associated with various proteins including enzymes under high temperature stress in leaf, fibrous root and storage root tissues. Tissue-specific responses, with both common and unique cellular responses were observed among the tissues. Pathway analysis revealed the differential regulation of genes involved in DNA replication, metabolism, transport, signaling, and stress response during high temperature stress. Six genes viz., DnaJ-domain protein (IpDnaJ), nuclear protein (IpELF5), heat shock protein 90.1 (IpHsp90.1), ABC transporter (IpABC) hydrolase (IpNUDX1) and alternative oxidase 1a (IpAO1a), were up-regulated in the leaf, fibrous root and tuberous root tissues. These six genes might play an important role in imparting high temperature stress tolerance in the leaf, fibrous root and tuberous root tissues of sweet potato. The information generated provides valuable insights on leaf, tuberous root and fibrous root tissue-specific high temperature stress-responsive genes in sweet potato. These datasets will be helpful in selecting candidate genes and pathways for further functional and genomic analyses, facilitating the genetic improvement of sweet potato with enhanced stress tolerance.