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http://krishi.icar.gov.in/jspui/handle/123456789/82980
Title: | Effect of prostaglandin producing modulators on in vitro growth of buffalo uterine epithelial cells |
Other Titles: | Not Available |
Authors: | Nandi S Mondal S Reddy IJ |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute of Animal Nutrition and Physiology |
Published/ Complete Date: | 2012-01-01 |
Project Code: | Not Available |
Keywords: | prostaglandin in vitro growth of buffalo uterine epithelial cells |
Publisher: | Elsevier Ltd |
Citation: | Nandi S, Mondal S and Reddy IJ. 2012. Effect of prostaglandin producing modulators on in vitro growth of buffalo uterine epithelial cells . Theriogenology, 77:1014-1020. |
Series/Report no.: | Not Available; |
Abstract/Description: | Studies were conducted to examine the effect of seven prostaglandin producing modulators on the in vitro growth of uterine epithelial cells in buffalo. The uterine epithelial cells isolated from slaughtered buffaloes were cultured in media containing (a) Lipopolysaccaride (LPS): 0, 0.01, 0.1, 1, 10 and 100 μg/ml, (b) linoleic acid: 0, 0.01, 0.1, 1, 10 and 100 μg/ml, (c) linolenic acid: 0, 0.01, 0.1, 1, 10 and 100 μg/ml, (d) oxytocin: 0, 10, 100, 1,000, 10,000 and 100,000 nm, (e) tumor necrosis factor-α (TNF-α): 0, 0.05, 0.5, 1, 2.5 and 5 nm, (f) progesterone: 0.1, 10, 25, 50, 75 and 100 nM, and (g) estradiol: 0, 2.5, 5, 10, 20 and 50 nM. The control medium consisted of RPMI-1640 plus 10% bovine fetal serum. The growth of uterine epithelial were measured in terms of viability, cell number increment and monolayer formation. Results suggested that the growth of uterine epithelial cells were significantly (P < 0.05) higher in media containing 10 μg/ml, 10 μg/ml, 1 nm and 10 μg/ml linoleic acid, linolenic acid, TNF-α and LPS, respectively compared to control and lower doses used. Progesterone, estradiol and oxytocin did not significantly (P > 0.05) increase the growth of uterine epithelial cells. In conclusion, the growth of uterine epithelial cells increased when exposed to modulators in the order of linoleic acid ≥ linolenic acid ≥ LPS ≥ TNF-α > progesterone > estrogen > oxytocin. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Theriogenology |
Volume No.: | 77 |
Page Number: | 1014-1020 |
Name of the Division/Regional Station: | Animal Physiology |
Source, DOI or any other URL: | 10.1016/j.theriogenology.2011.09.017 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/82980 |
Appears in Collections: | AS-NIANP-Publication |
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