Genotyping of Myostatin gene in white turkey using PCR- RFLP

Abstract

Myostatin gene plays an important role in limiting skeletal muscle growth in livestock and poultry species. The purpose of the present study was to identify the polymorphism in Myostatin gene and to associate the genetic variants with growth and feed efficiency traits in White turkey. Genomic DNA was isolated from 83 poults using Phenol: Chloroform extraction method. Polymerase chain reaction (PCR) was used for amplification of 611 bp fragment from intron 1 of Myostatin locus. The amplified products were analyzed for Restriction fragment length polymorphism (RFLP) using Hinf I restriction enzyme and the resulted fragment were visualized on 2% agarose gel stained with ethydium bromide under gel documentation system. PCR- RFLP analysis revealed monomorphism. Since no genetic variability for Myostatin gene was observed in the population, it was not possible to perform analysis studies with growth and feed efficiency traits. Lack of variability indicates the conserved nature of the gene.