Enhanced metabolite yield with compensatory biomass reduction revealed by moisture stress induction in Centella asiatica (L.)

Abstract

The exposure to any kind of stress tends to accelerate the secondary metabolism in medicinal plants increasing the production of secondary metabolites. The present investigation was undertaken to study the effect of moisture stress (100, 75, 50, 25 and 10% pot capacity) and control (as without plant) on growth, yield and metabolite content of Centella asiatica var. ‘Arka Prabhavi’, for two growing seasons under polyhouse conditions. Results revealed that moisture stress treatments had a significant effect on all observed growth and yield traits. Plants maintained at 100% PC exhibited luxurious vegetative growth with maximum leaf length (6.28 cm), leaf breadth (8.14 cm), petiole length (22.32 cm) and fresh biomass yield (164 g/pot). Cumulative water transpired and water use efficiency of the plants was also observed to be maximum at 100% PC. In contrary to biomass yield, increased asiaticoside (1.864%, 1.892%), madecassoside (2.856%, 3.382%) and total triterpenoid content (5.356%, 5.578%) at higher moisture stress levels of 75% and 50% PC, respectively, was observed. Hence, it is appropriate to grow Centella either at 100% or 75% PC to get optimum biomass and metabolite yield on a commercial scale.