KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/8583| Title: | Development of a probe based real time PCR assay for detection of bovine herpes virus-1 in semen and other clinical samples. |
| Other Titles: | Not Available |
| Authors: | Chandranaik BM Rathnamma D Patil SS Kovi RC Dhawan J Ranganatha S Isloor S Renukaprasad C Prabhudas K |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
| Published/ Complete Date: | 2013-06-01 |
| Project Code: | Not Available |
| Keywords: | Bovine herpes virus-1 Indirect ELISA Real time PCR Virus isolation |
| Publisher: | Pubmed |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | This study describes development of a TaqMan probe based real time PCR assay that can detect BoHV-1 of as low as 0.001 TCID50/0.1 ml in clinical samples, its comparative evaluation with indirect ELISA and virus isolation for detection of Bovine herpes virus-1 (BoHV-1) in semen and swab clinical samples. For this study, we collected samples from 212 animals (cattle and buffaloes) comprising 91 bulls and 121 females. Avidin-biotin ELISA employed on serum samples from 212 animals revealed 74 as seropositive for BoHV-1. On inoculation of semen/swabs on MDBK cell line, nine samples yielded cytopathic changes characteristic of herpes viruses. The isolates were confirmed by VNT and a conventional PCR. A real time PCR assay was standardised by designing a new set of TaqMan probe and primers targeting a 71 bp region on gB gene of the virus. The assay detected viral antigen in 21 seropositive and 14 seronegative animals, emphasizing the relevance of serology in BoHV-1 diagnosis, particularly in breeding stations. Further, real time PCR assay was 100 % sensitive and 87.19 % specific compared to virus isolation in detection of the BoHV-1 in clinical samples. The assay was validated at reputed national laboratories, with a sensitivity of ≥99 %. |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Virus Disease (Indian Journal of Virology) |
| NAAS Rating: | 5.95 |
| Volume No.: | 24(1) |
| Page Number: | 16-26 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.1007/s13337-012-0112-1 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/8583 |
| Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.