KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/9272| Title: | Duplex PCR for specific detection of Escherichia coli and its differentiation from other Enterobacteriaceae. |
| Other Titles: | Not Available |
| Authors: | Mitra SD Wilfred AD Tewari R Nimita VC Bhuvana M Krithiga N Shome BR Rahman H |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
| Published/ Complete Date: | 2015-08-01 |
| Project Code: | Not Available |
| Keywords: | Duplex polymerase chain reaction Enterobacteriaceae Escherichia coli |
| Publisher: | Indian Council of Agricultural Research |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Escherichia coli is a normal inhabitant of gastrointestinal tract of humans and animals and one of the mostimportant causes of bovine mastitis. Definitive identification of E. coli from other members of Enterobacteriaceaeremains ambiguous. The present study describes a duplex PCR, targeting 2 housekeeping genes, the lacy (lactosepermease) and phoA (alkaline phosphatase) for the reliable detection of E. coli that enables its differentiation frombiochemically and phylogenetically related bacteria. The assay was evaluated employing 19 ATCC (American typeculture collection) reference strains of Enterobacteriaceae family. Validation of the assay with E. coli (154) isolatedfrom milk and faeces rendered the assay to be specific. The results suggest that the technique can be used foraccurate detection of E. coli and thus can be adapted for testing bacteriological safety of milk, for field applications,and in laboratories handling clinical samples. This PCR (polymerase chain reaction) can successfully distinguishE. coli including E. coli O157 from Shigella spp and other related enterobacteria, emphasizing its relevance andutility in studies related to E. coli infection |
| Description: | Not Available |
| ISSN: | 0367-8318 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Animal Sciences |
| NAAS Rating: | 6.28 |
| Volume No.: | 85(8) |
| Page Number: | 832-835 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/50931 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/9272 |
| Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| RituSM1 (1).pdf | 626.9 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.