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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/9273
Title: | Streptococcus uberis ST439 and ST475 induce differential inflammatory responses in a mouse intramammary infection model |
Other Titles: | Not Available |
Authors: | Mitra SD Shome BR Bhuvana M Velu D Banerjee A Bankar K Ghosh SK Santra S Suresh KP Rahman H |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
Published/ Complete Date: | 2016-07-10 |
Project Code: | Not Available |
Keywords: | Immune response Mastitis Mice model S. uberis |
Publisher: | Elsevier |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Streptococcus uberis causing mastitis is a growing challenge to the dairy industry. Molecular, epidemiological and population structure studies have revealed clonal diversity among the infecting strains. In this study, mouse intramammary infection model was used to uncover the host immune response to two epidemiologically important live strains of S. uberis (SU1and SU2) obtained from subclinical case of mastitis possessing specific and unique multi locus sequence types (ST), pulsed field gel electrophoresis (PFGE) pulsotypes and virulence profiles. Temporal (2h, 4h, 8h, 12h, 24h and 48h) expression of key inflammatory mediators (IL2, IL4, IL6, IL12, TNFα, IFNγ, GMCSF, TLR2, TLR4, TLR9, TLR11, TLR12, CD14, IL1β, RANTES, Lactoferrin, and CXCl1) by reverse transcription and probe-based quantitative real-time PCR showed relative mRNA levels higher (p<0.05) in response to SU2 compared with SU1 with 24h PI serving as a critical point for the deviating behavior (SU1 versus SU2). Further employing the predicted biological processes under the influence of this pool of tested genes, the delineation of gene regulatory networks suggested SU1-favoring its persistence in the host environment; in contrast, SU2-which elevated gene expression indicating towards pathogen clearance or immune surveillance. This study suggested how these unique strains could manipulate the host immune response to influence the severity of mastitis; our results expand the available information on host pathogen interaction and provide a firm foundation needing further investigations to gain control over this pathogen |
Description: | Not Available |
ISSN: | 1596-5996 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Gene |
NAAS Rating: | 8.98 |
Volume No.: | 585(2) |
Page Number: | 247-255 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | 10.1016/j.gene.2016.03.054. |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/9273 |
Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
File | Description | Size | Format | |
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1-s2.0-S037811191630244X-main.pdf | 1.12 MB | Adobe PDF | View/Open |
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