General Part-1

Technology Code:-	:	201609839972187
Organization Details...
Subject Matter Division	:	{{smdOb.smdName}}
Organization Name	:	{{orgOb.orgName}} ,{{orgOb.City}}
AICRP name if any (AICRP)	:	All Not Applicable {{aicrpOb.aicrpName}}
Principal Inventor	:	Dr. Periyasamy Panneerselvam
Principal Inventor Designation:	:	Principal Scientist
Principal Inventor Email	:	panneerselvam.p@icar.gov.in
Principal Inventor Address	:	Microbiology, Crop Production Division.ICAR-NRRI, Cuttack 753006
Co-Inventor Name	:	Laxuman Sharma, A. K. Nayak, Ansuman Senapati, S. R. Prabhukarthikeyan, Upendra Kumar, Anjani Kumar, Guru Prasana Pandi, Debasis Mitra, A. Anandan and P. K. Nayak
Technology Name	:	Microbial consortium (MC) for pest and disease management in rice-horticulture based cropping system in Sikkim
Technology Details..
Major resource	:	All Not Applicable {{majorOb.majreName}}
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Technology Related To	:	All Not Applicable {{techrela.tr_name}}
Complete Details of Technology:	:
The following three bacterial strains (B. subtilis BioC-WB (NAIMCC-B-02285), B. luciferensis K2 (NAIMCC-B-02286) and B. amyloliquiefacies K12 (NAIMCC-B-02288) were used for microbial consortium preparation. All the selected bacterial strains were assessed for their antagonistic potential against some important plant pathogenic fungi of rice viz. Sclerotium rolfsii (seedling blight), Fusarium proliferatum (sheath rot) and Rhizoctonia solani (sheath blight). All the three bacterial strains have excellent antagonistic potential against above said plant pathogens. All the three strains have also good larvicidal activity against rice leaf folder with minimum cell load in the range of 2 to 2.97 x 104 CFU ml-1. Application of microbial consortium @ 5.0 lit ha 1 was found better performance under field conditions and resulted 16.0-22.0 % yield improvement in different vegetable and rice crops compared to framer practices under Sikkim condition.
Brief Description of Technology Including Salient Features:
Microbial consortium is liquid based formulation containing three efficient bacterial strains. Following three bacterial strains viz., B. subtilis BioC-WB (NAIMCC-B-02285), B. luciferensis K2 (NAIMCC-B-02286) and B. amyloliquiefacies K12 (NAIMCC-B-02288) were multiplied in nutrient broth and incubated till they attain log phase (i.e 36- 48 h,) with a cell load of 109 -1010 cells ml-1 and, then used for consortium preparation. The liquid microbial consortium was prepared by mixing the log phase cultures (109 -1010cells ml-1) of BioC-WB, K2 and K12 with equal proportion. After preparation, osmo-protectants (glycerol and polyvinyl pyrolidone) were added in different proportion and mixed gently. After mixing, the prepared consortium was stored in air tight plastic container. Assessment of shelf life under room condition indicated that this product can be stored without any deterioration up to six months. Microbial consortium (MC) were evaluated in different vegetable crops viz., broccoli, brinjal, cabbage, capsicum, okra, tomato and rice under greenhouse and field condition at Sikkim condition. This technology developed by using native bacterial strains from Sikkim soil, hence adoptability and colonization in the rhizosphere of plants are excellent. Salient feature: • Application of microbial consortium @ 5.0 lit ha 1 was found better performance under field conditions and resulted 16.0-22.0 % yield improvement in different vegetable and rice crops compared to framer practices under Sikkim Condition. • Improves microbial properties in the rhizosphere of crop plants • Protects plants from soil borne fungal pathogens and insects like rice leaf folder • Improves the plant growth and vigour of plants
Benefits/Utility	:
Application of microbial consortium @ 5.0 lit ha 1 was found better performance under field conditions and resulted 16.0-22.0 % yield improvement in different vegetable and rice crops compared to framer practices under Sikkim condition.
Precaution With The Technology	:	• Wear protective gear (goggles, masks, gloves). • Keep the product beyond the reach of children. • Avoid inhalation and skin contact during the mixing • Don’t eat / drink / smoke during the mixing process • In case of contact with eyes: Flush with water liberally for 20 minutes • After the work, wash hands with soap water • Microbial consortium should not be mixed with insecticide, fungicide, herbicide and fertilizers
Time Of Application Use	:	Basal application at the time of field preparation. Seed treatment followed by soil drenching after 7 days of transplanting
How To Use	:
Vegetables Seed treatment: 10-20 ml inoculums per 100 g seeds • Around 10-20 ml inoculums are sufficient to treat 100 g vegetable seeds. Take the required seed materials in small clean polythene bag and add sufficient inoculum along with 0.1 % carboxy methyl cellulose (sticking agent), then bag should be closed and squeezed for 3-4 minutes until all the seed are uniformly wetted. After through coating, the bag is opened and the seed is dried under the shade for 30 minutes. If we want to treat for large amount of seeds, big containers like plastic bucket can be used. The microbial treated seeds should be sown immediately after shade drying. Soil Drenching: 2.0 lit inoculums per acre • Consortium should be mixed with water @ 25 ml/ litre and then applied near the root zone on 5-10 days after transplanting. Root dipping: 500 ml inoculums per acre seedlings • The required quantity of inoculums should be mixed with 50 litres of water and the roots of seedlings should be immersed for half-an-hour before transplanting. Main field application: 2.0 lit inoculums per 500 kg FYM enrichment • For the main field application for one acre of land, 2-liter microbial consortium should be mixed with 500 kg of FYM and applied near the root zone of standing crop. Alternatively, it can be applied basally at the time of land preparation. Spread the compost in shaded place and uniformly sprinkle the diluted inoculums (one lit inoculums mixed in 20 L water) over the compost. Mix the inoculums thoroughly, after mixing, heap the compost and allow for 1-2 days for further multiplication. The enriched compost should be broadcasted in the field or it should be applied near the root zone of standing crop. Rice Seed treatment: 500 ml inoculums per one acre • Around 500 ml inoculums are sufficient to treat the rice seeds for one-acre field. Take the required seed materials in small clean polythene bag and add sufficient inoculum along with 0.1 % carboxy methyl cellulose (sticking agent), then bag should be closed and squeezed for 3-4 minutes until all the seed are uniformly wetted. After through coating, the bag is opened and the seed is dried under the shade for 30 minutes. If we want to treat for large amount of seeds, big containers like plastic bucket can be used. The microbial treated seeds should be sown immediately after shade drying. Root dipping: 500 ml inoculums per acre seedlings • The required quantity of inoculum (500 ml) should be mixed with 50 litre of water and the roots of seedlings should be immersed for half-an-hour before transplanting. Main field application: 2.0 litre inoculum per 500 kg FYM enrichment • For the main field application of one acre of land, 2-litre microbial consortium can be mixed with 500 kg of FYM and it can be applied basally at the time of land preparation. Spread the compost in shaded place and uniformly sprinkle the diluted inoculum (one litre inoculum mixed in 20 litre water) over the compost. Mix the inoculum thoroughly, after mixing, heap the compost and allow for 1-2 days for further multiplication. The enriched compost can be broadcasted in field.
Impact, If Adopted	:
Adoption this technology increases different vegetable and rice yield by 16.0-22.0 % under Sikkim condition. Protect the plant from various disease and insect under organic cultivation
Social Impact	:
This technology created the awareness among farming community on importance of microbial consortium for plant protection under organic cultivation. Now, farmers started using microbial consortium for different crops cultivation under Sikkim Condition.
Technology Spread	:
Under this programme, more than 50 farmers from different parts of Sikkim were trained for use of microbial consortium for their crops cultivation
TargetUsers/Stake holders	:	Farmers, State, Govt. Extension workers, KVKs, officials and SMS
Technology Contact..
Name	:	Director
Email	:	director.nrri@icar.gov.in , directorcrricuttack@gmail.com
Phone Number	:	0671-2367757
Fax Number	:	0671-2367663
Address	:	ICAR-National Rice Research Institute,Bidyadharpur,Cuttack-753006
Name	:	Dr. Periyasamy Panneerselvam
Email	:	panneerselvam.p@icar.gov.in

Technology Development Details Part-2

Project Details (Through which technology was developed)	:	Developing microbial consortium for horticultural crops in rice based cropping system to promote growth, nutrient uptake and diseases management in organic farming in Sikkim ( DBT sponsored twining mode project)
Time of Initiation Technology Development	:	1-2017
Time of Completion Technology Development	:	3-2020
Technology Validated by	:	Within ICAR
Technology Validation Details..
Subject Matter Division	:	{{smdOb.smdName}}
Organization Name(if within ICAR)	:	ICAR-National Rice Research Institute,Cuttack
Organization Name(if outside ICAR,Please enter)	:
Year of Release/Adoption(YYYY)	:	3-2019

Applies To(Regional Differentiation)Inform Part-3

Location...
Zone(As per the planning commission)	:	All Not Applicable {{zone.planningzoneName}}
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AgroEcological Zone(NBSS & LUP)	:	All Not Applicable {{agrozone.nbssaerName}}
AgroEcological Sub Zone(NBSS & LUP)	:	All Not Applicable {{agrosubzone.nbssaesrName}}
State Name	:	All Not Applicable {{state.stateName}}
District Name	:	All Not Applicable {{dist.distName}}
Soil Type/Resource Type..
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Commodity Details..
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Publication Related To Technology Part-4

Publication Related To Technology Part-4

Research Paper information..

1. Periyasamy Panneerselvam, Ansuman Senapati, Upendra Kumar, Laxuman Sharma, Pinky Lepcha, SR Prabhukarthikeyan, Afrin Jahan, Chidambaram Parameshwaran, Guru Prasana Pandi Govindharaj, Srikanta Lenka, Prafulla Kumar Nayak, Debasis Mitra, Mahapatra Smruthi Sagarika, Sugitha Thangappan and Utthandi Sivakumar (2019 ). Antagonistic and plant-growth promoting novel Bacillus species from long-term organic farming soils from Sikkim, India , 3 Biotech , 9., 11., Springer International Publishing.

Technical Bulletin information..

1. Periyasamy Panneerselvam, Laxuman Sharma, A.K. Nayak, Ansuman Senapati, Jigmi Tshering Bhutia, S.R. Prabhukarthikeyan, Upendra Kumar, Anjani Kumar, Guru Prasanna Pandi, Debasis Mitra, A. Anandan and P.K. Nayak (2020). Development of microbial consortium for plant growth promotion, nutrient and disease management in rice horticulture based cropping system in Sikkim., Director, ICAR-National Rice Research Institute, Cuttack-753006, Odisha, India, 28.