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http://krishi.icar.gov.in/jspui/handle/123456789/10206| Title: | Parasitological and molecular detection of bovine babesiosis in endemic areas of Karnataka state. |
| Other Titles: | Not Available |
| Authors: | Vijaykumar K U Puttalakshamma G C D’Souza P E Sengupta P P Chandranaik B M Renukaprasad C |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Veterinary College, Bengaluru ICAR::National Institute of Veterinary Epidemiology and Disease Informatics |
| Published/ Complete Date: | 2017-04-01 |
| Project Code: | Not Available |
| Keywords: | Acridine orange staining Bovine babesiosis Babesia bigemina Giemsa Fluorescent PCR |
| Publisher: | Indian Council of Agricultural Research |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | A study was conducted to compare the specificity and sensitivity of parasitological methods (Giemsa and Fluorescent acridine orange staining) and PCR to detect the carrier status, subclinical and clinical babesiosis in cattle from endemic regions of Karnataka state in South India. Out of 148 cattle screened, 3 (2.03%), 7 (4.73%) and 16 (10.81%) were found positive for B. bigemina infection by Giemsa’s staining, acridine orange staining and PCR, respectively. The conserved primers for B. bigemina derived from SpeI-AvaI restriction fragment were used in PCR which amplified a fragment of 278 bp in the positive cattle blood and was 83.26% sensitive and 91.16% specific for detection of babesiosis in cattle. |
| Description: | Not Available |
| ISSN: | 2231-0916 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Animal Sciences |
| NAAS Rating: | 6.28 |
| Volume No.: | 87(4) |
| Page Number: | 422-426 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/69512 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/10206 |
| Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| BabesiacattleIJAS.pdf | 134.09 kB | Adobe PDF | View/Open |
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