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http://krishi.icar.gov.in/jspui/handle/123456789/10294
Title: | Development of EST-SSR markers and their utility in revealing cryptic diversity in safflower (Carthamus tinctorius L.) |
Other Titles: | Not Available |
Authors: | K.N. Yamini K. Ramesh V. Naresh P. Rajendrakumar K. Anjani V. Dinesh Kumar |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Oilseeds Research Acharya N.G. Ranga Agricultural University ICAR::Indian Institute of Millets Research |
Published/ Complete Date: | 2012-05-15 |
Project Code: | Not Available |
Keywords: | EST-SSR Safflower Polymorphism Transferability Diversity |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | With the aim of developing additional genomic resources in safflower, a set of 41,011 ESTs of safflower were mined for the presence of SSRs. 18,773 SSR containing ESTs (SSR-ESTs) were identified and were analyzed to remove redundant sequences leading to identification of 8,810 nonredundant SSR-ESTs (categorized into 6104 singletons and 2,706 contigs) having 13,085 non-redundant SSRs. The average number of non-redundant SSRs per ESTwas 0.32 and they predominantly consisted of dinucleotide (57.7 %), and trinucleotide (37.7%) repeat motifs. 500 primer pairs were designed for the non-redundant EST-SSRs of which, 151 were tested. 60 markers which gave robust amplicons, were validated in a set of 19 Carthamus lines. A subset of EST-SSR markers, having average polymorphism information content (PIC) ≥0.4 could precisely elucidate the pedigree relatedness among these lines. Further, these markers exhibited high cross-species transferability to five other wild species of Carthamus. The markers reported here would be a valuable addition to existing safflower marker resources aiding in hastening its improvement. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Journal of Plant Biochemistry and Biotechnology |
NAAS Rating: | 6.77 |
Volume No.: | 22(1) |
Page Number: | 90–102 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | DOI 10.1007/s13562-012-0115-4 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/10294 |
Appears in Collections: | CS-IIMilletsR-Publication |
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