KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/11308
Title: | Detection of Specific Polypeptide(s) Synthesized during the Sequential Stages of Differentiation in Dioscorea species |
Other Titles: | Not Available |
Authors: | Ashwani Kumar, Suresh Chand Goyal, Pooja Dhansu, Parvender Sheoran, Arvind Kumar, Ekta Verma, Jagdish Parshad and Anshuman Singh |
Author's Affiliated institute: | ICAR-Central Soil Salinity Research Institute, Karnal, Haryana, India |
Published/ Complete Date: | 2015-09-01 |
Project Code: | Not Available |
Keywords: | Dioscorea alata, Dioscorea deltoidea, callus, differentiation, polypeptides |
Publisher: | Not Available |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | The present investigation was aimed to detect the specific polypeptide(s) appeared during the sequential stages of differentiation. Among different explants, only nodal explants showed good results for callusing. Depending on the fresh and dry weight, best callus growth was observed on MS medium supplemented with NAA (2.5 mg/L) in Dioscorea alata and 2, 4-D (2.0 mg/L) in D. deltoidea, respectively. This callus was used for the regeneration. Roots differentiation was observed on MS medium + NAA (2.0 mg/L) + IBA (0.5 mg/L) and shoots on MS medium + BAP (2.0 mg/L) + NAA (0.5 mg/L) in D. alata while in D. deltoidea, roots on RT medium + IAA (1.0 mg/L) and shoots on RT medium + BAP (1.0 mg/L) + NAA (0.5 mg/L). Continuous decrease was seen in the total soluble protein during the differentiation in D. alata whereas in D. deltoidea, the protein content decreased upto initiation stage. Four root specific polypeptides (MW 25.56, 24.35, 19.13 and 18.2 kDa) and three shoot specific polypeptides (MW 53.7, 25.12 and 19.13 kDa) were synthesized during the differentiation in D. alata. Similarly, two root specific (MW 33.9 and 31.69 kDa) and one shoot specific (MW 16.98 kDa) polypeptide band were appeared during differentiation in D.deltoidea. |
Description: | Not Available |
ISSN: | 1516-8913 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Brazilian Archives of Biology and Technology |
NAAS Rating: | 6.58 |
Volume No.: | 58 (5) |
Page Number: | 667-675 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | http://dx.doi.org/10.1590/S1516-89132015050185 |
URI: | http://krishi.icar.gov.in/Publication/handle/123456789/11308 |
Appears in Collections: | NRM-CSSRI-Publication |
Files in This Item:
There are no files associated with this item.
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.