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http://krishi.icar.gov.in/jspui/handle/123456789/12063
Title: | Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.) |
Other Titles: | Not Available |
Authors: | Binay K. Singh, Divakar Nandan, Supriya Ambawat, Bhagirath Ram, Arun Kumar, Tawar Singh, H. S. Meena, Vinod Kumar, V. V. Singh, P. K. Rai, and Dhiraj Singh |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Directorate of Rapeseed Mustard Research, Sewar, Bharatpur - 321 303, Rajasthan, India Centre for Genetic Manipulation of Crop Plants, University of Delhi, South Campus, Benito Juarez Marg, New Delhi - 110 021, India. |
Published/ Complete Date: | 2015-05-21 |
Project Code: | Not Available |
Keywords: | White rust, marker-assisted selection, marker validation |
Publisher: | Canadian Journal of Plant Science |
Citation: | 2 |
Series/Report no.: | Not Available; |
Abstract/Description: | Singh, B. K., Nandan, D., Supriya, A., Ram, B., Kumar, A., Singh, T., Meena, H. S., Kumar, V., Singh, V. V., Rai, P. K. and Singh, D. 2015. Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.). Can. J. Plant Sci. 95: 939 945. Successful application of molecular markers in markerassisted pyramiding relies on effective determination of the target phenotype. In this respect, evaluation of the efficiency of markers for marker-assisted selection through cross-validation in different genetic backgrounds and in different populations is a crucial step. In the present study, the previously identified Arabidopsis-derived intron polymorphic (IP) markers At5g41560 and At2g36360, which were highly linked with AcB1-A4.1 and AcB1-A5.1, respectively, were validated in a set of 25 genotypes of Indian Mustard and in three different F2 populations. The relationships between the variation of PCR products of the two markers with the percent disease index (PDI) of the tested genotypes, and the co-segregation analysis of the markers with disease phenotype in F2 populations clearly indicated that At5g41560 and At2g36360 are genotype-nonspecific markers and are closely linked to white rust resistance loci AcB1-A4.1 and AcB1-A5.1, respectively. It also became evident from the present study that AcB1-A4.1 and an another white rust resistance locus Ac(2)t are likely the same gene locus. |
Description: | Not Available |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Canadian Journal of Plant Science |
NAAS Rating: | 6.85 |
Volume No.: | 95 |
Page Number: | 939-945 |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/12063 |
Appears in Collections: | CS-DRMR-Publication |
Files in This Item:
File | Description | Size | Format | |
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cjps-2014-215.pdf | 121.08 kB | Adobe PDF | View/Open |
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