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http://krishi.icar.gov.in/jspui/handle/123456789/12350| Title: | Development of EMA-2 recombinant antigen based enzyme-linked immunosorbent assay for seroprevalence studies of Theileria equi infection in Indian equine population |
| Other Titles: | Not Available |
| Authors: | Sanjay Kumar, Rajender Kumar, Ashok K. Gupta, Suresh C. Yadav, Sachin K. Goyal, Sandip K. Khurana, Raj K. Singh |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Equines |
| Published/ Complete Date: | 2013-08-30 |
| Project Code: | Not Available |
| Keywords: | Babesia equi ,Equine piroplasmosis,ELISA ,EMA-2 ,Seroprevalence ,Theileria equi |
| Publisher: | ELSEVIER |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Equine piroplasmosis is a tick-transmitted protozoan disease caused by Theileria equi and/or Babesia caballi. In the present study, we expressed a 53 kDa protein from the truncated EMA- 2 gene of T. equi (Indian strain) and developed EMA-2ELISA using this expressed protein. This ELISA is able to detect T. equi-specific antibodies in experimentally infected animals as early as 9 days post-infection. The assay developed was validated with the OIE recommended competitive ELISA (cELISA) on 120 serum samples and significant agreement (kappa = 0.93) was observed between results of both the ELISAs which indicates suitability of EMA-2ELISA for use in sero-diagnosis. Diagnostic sensitivity and specificity of EMA-2ELISA – as compared with cELISA – were 0.97 and 0.96, respectively. Analysis of 5651 equine serum samples – collected during 2007–2012 from 12 states of India representing eight agro-climatic zones – by EMA-2ELISA revealed 32.65% seroprevalence of T. equi in India. In conclusion, the EMA-2ELISA developed using the T. equi EMA-2 recombinant protein as antigen for detecting T. equi-specific antibodies has good diagnostic potential for sero-epidemiological surveys. |
| Description: | Not Available |
| ISSN: | 0304-4017 |
| Type(s) of content: | Journal |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Veterinary Parasitology |
| NAAS Rating: | 8.16 |
| Volume No.: | 198 |
| Page Number: | 10– 17 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://dx.doi.org/10.1016/j.vetpar.2013.08.030 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/12350 |
| Appears in Collections: | AS-NRCE-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Vet Para ELISA 2013 paper Author's Copy.pdf | 1.16 MB | Adobe PDF | View/Open |
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