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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/12354
Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Sandeep Kumar, Naresh K. Rakha, Liza Goyal, Parveen Goel, Rajender Kumar, Ashok Kumar and Sanjay Kumar | en_US |
dc.date.accessioned | 2018-11-20T11:04:01Z | - |
dc.date.available | 2018-11-20T11:04:01Z | - |
dc.date.issued | 2015-07-06 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | 0047-1917 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/12354 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Theileria equi merozoite surface antigens have been an important candidate for development of diagnostics. We developed ELISA based on EMA-1 recombinant antigen, so as to widen our diagnostic confidence in detection of antibodies against T. equi in serosurveillance studies. The 547 bp EMA-1 gene fragment encoding high hydrophilic antigenic region was expressed with glutathione-S-transferase tag in prokaryotic system and purified protein (43 kDa) was used for development of ELISA (EMA-1t/ELISA). The EMA-1t/ELISA clearly differentiated T. equi-infected from Babesia caballi-infected horse sera or normal horse sera. The results of the study were validated with previously developed EMA-2ELISA on serum samples of known T. equi infection status and a very high correlation (0.93) was recorded between the relative percent positivity (RPP) values. Further diagnostic sensitivity of EMA-1t/ELISA was 0.92 while specificity was 1.0, indicating its suitability for sero-epidemiological studies. This assay was applied on serum samples (n = 240) collected from field horses in northern part of India. High sero-prevalence of T. equi antibodies were diagnosed in serum samples collected from Haryana state (74%) and Uttarakhand state (36.31%). Results of this study suggested that the 43 kDa EMA-1 expressed protein could be a reliable immunodiagnostic antigen in ELISA for T. equi sero-prevalence studies. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Japanese Journal of Veterinary Research | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Theileria equi, EMA-1, diagnosis, ELISA, Babesia equi. | en_US |
dc.title | Diagnostic application of recombinant equine merozoite surface antigen-1 in elisa for detection of Theileria equi specific antibodies | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Journal | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Japanese Journal of Veterinary Research | en_US |
dc.publication.volumeno | 63(3) | en_US |
dc.publication.pagenumber | 129-137 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.14943/jjvr.63.3.129 | en_US |
dc.publication.authorAffiliation | ICAR::National Research Centre on Equines | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.32 | en_US |
Appears in Collections: | AS-NRCE-Publication |
Files in This Item:
File | Description | Size | Format | |
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JJVR Sandeep EMA-1 ELISA.pdf | 392.78 kB | Adobe PDF | View/Open |
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