Amplification of Microsatellite Loci Using Genomic DNA of Trypanosoma evansi Isolates from Different Geographical Areas of India

Abstract

Surra is an economically important disease of livestock caused by an extracellular haemoprotozoan parasite, Trypanosoma evansi. In this study, genomic DNA was extracted from six Trypanosoma isolates collected from different geographical areas, and twelve microsatellite loci were successfully amplified in polymerase chain reaction (PCR) with selected microsatellite primers. For most of the microsatellite loci, the PCR amplicon size in Indian isolates was found similar to that of Trypanosoma brucei TREU927, except for three microsatellite loci (TB11/1, TB10/1 and TB7/12). For the microsatellite loci TB11/1, TB10/1 and TB7/ 12, the PCR amplicon size in T. evansi isolates was found to be 160, 210 and 200 (bp), respectively. Similar amplicon size patterns using twelve microsatellite primers were observed in all six Indian T. evansi isolates used in this study.