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http://krishi.icar.gov.in/jspui/handle/123456789/12549| Title: | PCR-based Identification of Trypanosoma evansi targeting Internaltranscribed Spacer 1 of rDNA in experimentally infected Mice |
| Other Titles: | Not Available |
| Authors: | Shashi Kant Kankar, Sachin Kumar Goyal, Parvati Sharma, Deepak Kumar Gaur, Souti Prasad Sarkhel and Rajender Kumar |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Equines ICAR::Indian Veterinary Research Institute |
| Published/ Complete Date: | 2015-02-04 |
| Project Code: | Not Available |
| Keywords: | Trypanosma evansi, Surra, ITS-1, PCR, rDNA, Equine |
| Publisher: | Indian Journals.com |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Trypanosoma evansi (T. evansi) is a causative agent of disease called surra, affecting wide range of domestic and wild animals. In this study, a PCR assay was developed using primers targeting ITS-1 region flaking between 5.8 S and 18 S subunits of rRNA. The test was employed using serially diluted DNA extracted from purified trypanosomes ranging from 200 ng/μl to 0.00002 pg/μl and T. evansi infected mice blood ranging from 350 ng/μl to 0.00035 ng/μl. The diagnostic sensitivity of PCR assay was found to be 0.2 pg/μl and 0.035 ng/μl with purified parasite DNA and infected mice blood DNA, respectively. |
| Description: | Not Available |
| ISSN: | 0973-9149 |
| Type(s) of content: | Journal |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Immunology and Immunopathology |
| Volume No.: | 15 |
| Page Number: | 210-214 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.5958/0973-9149.2014.01070.3 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/12549 |
| Appears in Collections: | AS-NRCE-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 12-Kankar et al 2014-1.pdf | 701.93 kB | Adobe PDF | View/Open |
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