Semen quality parameters of mithun semen preserved at liquid state (5°C)

Abstract

A study was conducted to assess the effect of liquid semen preservation on semen quality parameters (SQPs) such as forward progressive motility, livability, acrosomal integrity and total morphological abnormality at refrigerated temperature in mithun. Good quality ejaculates (50) were collected and preserved at refrigerated temperature (5°C) for 72 h using tris egg yolk citrate based extender (TEYCE). Seminal attributes, viz. colour, consistency, volume, mass activity and sperm concentration of fresh semen samples were creamy white, medium, 1.8±0.08 ml, 3.4±0.09 (5 point scale) and 478±15 (×106/ml), respectively. SQPs were assessed after initial dilution, after final dilution and up to 72 h at 6 h intervals. The progressive motility, live sperm with intact acrosome decreased significantly over the period of storage and were below 20% after 48 h of storage. Total morphological abnormalities increased significantly at the time of 30% progressive motility. The study concluded that the mithun sperm can be preserved in tris based egg yolk extender in liquid storage at refrigeration temperature (5°C) for 36 h.