KRISHI
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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/12908
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | V Venkateswarlu | en_US |
dc.contributor.author | J Sridhar | en_US |
dc.contributor.author | R Baswaraj | en_US |
dc.contributor.author | A Jeevalatha | en_US |
dc.contributor.author | K Malik | en_US |
dc.contributor.author | A Bhatnagar | en_US |
dc.contributor.author | Neelum K | en_US |
dc.contributor.author | S Sanjeev | en_US |
dc.contributor.author | M Nagesh | en_US |
dc.contributor.author | BP Singh | en_US |
dc.contributor.author | SK Chakrabarti1 | en_US |
dc.date.accessioned | 2018-11-23T05:46:07Z | - |
dc.date.available | 2018-11-23T05:46:07Z | - |
dc.date.issued | 2016-08-11 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/12908 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Myzus persicae is the principal vector of economically important potato viruses, especially Potato virus Y and Potato leaf roll virus. Uniplex and duplex reverse transcription polymerase chain reaction (RT-PCR) assays were standardized and validated to detect PVY and PLRV in field collected populations of M. persicae using primers specific to coat protein. Results revealed that an annealing temperature of 62°C was found optimum for amplification of both viruses. Uniplex assay developed in this study could detect PVY in M. persicae samples of Modipuram, Jalandhar, Solan, Patna and Shillong. Duplex assay could successfully detect both viruses in M. persicae samples of Kodaikanal and Shillong. PCR products of PVY and PLRV were direct sequenced and got desired nucleotides of 380 and 492 bp, respectively which further confirmed the robustness of the protocols. Therefore, these protocols could be helpful to detect PVY and PLRV in M. persicae for healthy potato seed production. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Not Available | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Coat protein, COI, Myzus persicae, RT-PCR, PVY, PLRV | en_US |
dc.title | UNIPLEX AND DUPLEX RT-PCR PROTOCOLS FOR DETECTION OF PVY AND PLRV IN APHIDS FROM POTATO FIELDS | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Potato Journal (Journal of Indian Potato Association) | en_US |
dc.publication.volumeno | 43(2) | en_US |
dc.publication.pagenumber | 146-152 | en_US |
dc.publication.divisionUnit | Division of Crop Protection | en_US |
dc.publication.sourceUrl | Not Available | en_US |
dc.publication.authorAffiliation | ICAR-CENTRAL TOBACCO RESEARCH INSTITUTE | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | Not Available | en_US |
Appears in Collections: | CS-CTRI-Publication |
Files in This Item:
File | Description | Size | Format | |
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Uniplex_and_Duplex-V__Venkateswarlu_et_al._15-12-2016.pdf | 557.05 kB | Adobe PDF | View/Open |
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