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http://krishi.icar.gov.in/jspui/handle/123456789/13201| Title: | Temporal expression of cumulus cell marker genes during in vitro maturation and oocyte developmental competence |
| Other Titles: | Not Available |
| Authors: | Dhali A Javvaji PK Kolte AP Francis JR Roy SC Sejian V |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-NIANP and others |
| Published/ Complete Date: | 2017-07-01 |
| Project Code: | BSA-4005/2013-14 |
| Keywords: | Cumulus cells Embryo Gene expression In vitro development Oocyte Ovine |
| Publisher: | Not Available |
| Citation: | Dhali A, Javvaji PK, Kolte AP, Francis JR, Roy SC and Sejian V. 2017. Temporal expression of cumulus cell marker genes during in vitro maturation and oocyte developmental competence. Journal of Assisted Reproduction and Genetics, 34:1493-1500. |
| Series/Report no.: | Not Available; |
| Abstract/Description: | PURPOSE:Cumulus cells (CC) play important roles in oocyte development and cumulus expressed genes can be used as markers for oocyte quality. This study aimed to investigate temporal changes in the expression of cumulus marker genes during oocyte maturation as possible biomarkers of embryo developmental competence in ovine. METHODS: Gene expression was assessed in the CC of the BCB+ (developmentally competent) and BCB- (developmentally poor) oocytes at 0, 12, and 24 h of in vitro maturation (IVM). Further, the association between the temporal cumulus gene expression and in vitro oocyte and embryo development was assessed. RESULTS: The maturation and blastocyst formation rates were found significantly greater for the BCB+ than the BCB- oocytes. At the 0 h of IVM, a significant upregulation in the expression of PTGS2, STAR, SDC2, LHR, FGF2, BCL2, IL7RA, HSPA1A, and IFNT was observed in the CC of the poor (BCB-) as compared to the competent (BCB+) oocytes. In contrast, it was observed that as maturation progressed, the cumulus expression of most of the favorable genes was reduced and was found significantly downregulated at the completion of IVM in the poor as compared to the competent oocytes. CONCLUSIONS: The study revealed noticeable differences in the cumulus gene expression profile at different stages of IVM between ovine oocytes of differential developmental ability. The results indicated that the loss of cumulus gene expression along the maturation period in the poor oocytes was related to their intrinsic poor quality in the ovarian follicle. |
| Description: | Not Available |
| ISSN: | 1058-0468 |
| Type(s) of content: | Research Paper |
| Sponsors: | NFBSFARA/BSA-4005/2013-14 |
| Language: | English |
| Name of Journal: | Journal of Assisted Reproduction and Genetics |
| NAAS Rating: | 8.83 |
| Volume No.: | 34 |
| Page Number: | 1493-1500 |
| Name of the Division/Regional Station: | BEES DIV |
| Source, DOI or any other URL: | 10.1007/s10815-017-0998-z. |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13201 |
| Appears in Collections: | AS-NIANP-Publication |
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