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http://krishi.icar.gov.in/jspui/handle/123456789/13237
Title: | Suppression of growth and endopeptidases of red palm weevil, Rhynchophorus ferrugineus (Olivier), infesting coconut using proteinase inhibitors. |
Other Titles: | Not Available |
Authors: | Not Available Josephrajkumar, A., Chandrika Mohan and Chaturvedi, V.K. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-CPCRI, Regional Station, Kayamkulam |
Published/ Complete Date: | 2017-02-18 |
Project Code: | 1000765041 |
Keywords: | Rhynchophorus ferrugineus, gut proteinase, proteinase inhibitors, aprotinin, soybean trypsin inhibitor |
Publisher: | Association for Advancement of Entomology |
Citation: | Entomon |
Series/Report no.: | Not Available; 41(4);283-293 |
Abstract/Description: | Investigations on luminal proteinases of grubs of red palm weevil, Rhynchophorus ferrugineus (Olivier) [Curculionidae: Coleoptera] infesting coconut revealed the presence of two endodpeptidases viz., trypsin (BApNA-ase activity) and elastase-like chymotrypsin (SAAPLpNA-ase activity) in all stages of larval development. Highest activity of these proteinases coincided with the active feeding stage (mid-larval stage) of the insect. Results indicated that 50 g of aprotinin, 50 g of Soybean Trypsin Inhibitor (SBTI) and 1700 g of Phenyl Methyl Sulphonyl Fluoride (PMSF) inhibited trypsin activity of R. ferrugineus by 77.4%, 63.1% and 55.9%, respectively. Serine proteinase inhibitors viz., aprotinin (50 g), SBTI (50 g) and PMSF (1700 g) had a marginal reduction of elastase-like chymotrypsin activity of R. ferrugineus by 32%, 14% and 11%, respectively suggesting the serine nature of the proteinase. Aprotinin caused higher inhibition than SBTI and PMSF for both the proteinases studied. In vivo bioassay of 250 M aprotinin on coconut petiole method using early stage grubs of R. ferrugineus indicated a significant weight loss of 18.9% due to incorporation of serine proteinase inhibitor, aprotinin in a period of 120 h. Possibility of using serine proteinase inhibitor, aprotinin in the management of R. ferrugineus was suggested. Not Available |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Entomon |
NAAS Rating: | 4.69 |
Volume No.: | 41(4) |
Page Number: | 283-293 |
Name of the Division/Regional Station: | Division of Crop Protection |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13237 |
Appears in Collections: | HS-CPCRI-Publication |
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