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http://krishi.icar.gov.in/jspui/handle/123456789/13275
Title: | Diversity of culturable and unculturable gut bacteria associated with field population of Spodoptera litura (Fab.) |
Other Titles: | Not Available |
Authors: | Sanjay Yalashetti Mahesh Shankarappa Yandigeri Onkarappa Rudrappa Mohan Muthugounder Sivakumar Gopalasamy |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR-National Bureau of Agricultural Insect Resources, P.B No. 2491, HA Farm Post, Bellary Road, Bengaluru-560024, Karnataka, India Sahyadri Science College, Vidya Nagar, Shivamogga, India-577 203 |
Published/ Complete Date: | 2017-08-29 |
Project Code: | OXX02755 |
Keywords: | diversity, gut bacteria, insecticide degradation, Spodoptera litura |
Publisher: | Academy for Environment and Life Sciences, India |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Polyphagus crop pest Spodoptera litura (Fab.) harbor various symbiotic microorganisms providing several benefits to the host with various types of interactions. The field collected and laboratory reared populations of S. litura were subjected to isolation of culturable endosymbiotic gut microflora and 16S rDNA sequence based identification. Enterobacter cloacae, Lysinibacillus macroides, Pseudomonas stutzeri, Staphylococcus saprophyticus and S. sciuri were identified as culturable gut bacterial flora These bacteria possessed enzymatic activity aiding in digestion. Apart from this, E. cloacae, P. stutzeri and S. saprophyticus were found common in both rhizospheric soil and insect gut possibly indicating their movement from soil to plants and further to the insects through feeding. Metagenomic studies by traditional cloning of 16S rDNA resulted in identification of uncultured Enterococcus sp., Parvimonas sp., Erysipelatoclostridium sp., Dysgonomonas sp. and Gilliamella sp. from the field population. An attempt was made to study the endosymbiont Wolbachia using wolbachia surface protein gene revealed absence of Wolbachia infection. Ability of S. litura gut bacteria for degradation of insecticides leading to the insecticide resistance of the host was studied by in vitro microbial degradation of insecticide imidacloprid. Luxurious growth of gut symbiont Lysinibacillus macrolides SPL-1 in insecticides amended M9 medium implied the utilization of insecticides as carbon sources for growth and its possible involvement in degradation of insecticides. |
Description: | Not Available |
ISSN: | Online ISSN 2277-1808 |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Bulletin of Environment, Pharmacology and Life Sciences |
NAAS Rating: | Not Available |
Volume No.: | 6(2) Special issue (2) 2017 |
Page Number: | 441-451 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13275 |
Appears in Collections: | CS-NBAIR-Publication |
Files in This Item:
File | Description | Size | Format | |
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BEPLS 2017.pdf | 533.38 kB | Adobe PDF | View/Open |
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