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http://krishi.icar.gov.in/jspui/handle/123456789/13941| Title: | Expression and characterization of recombinant single chain beta-alpha equine chorionic gonadotropin in prokaryotic host |
| Other Titles: | Not Available |
| Authors: | Anuradha Bhardwaj*, Sanjay Kumar, Varij Nayan1, Parvati Sharma, Yash Pal and S.C. Yadav |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Research Centre on Equines |
| Published/ Complete Date: | 2018-03-20 |
| Project Code: | Not Available |
| Keywords: | eCG; Escherichia coli, Expression, Hormone, Pituitary, Protein, Recombinant |
| Publisher: | ARCC |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | The aim of present study was to produce recombinant eCG/pituitary glycoprotein in the Escherichia coli (E. coli) BL21C host cells and to test its diagnostic efficacy. This aim was achieved by optimizing its expression, purification as well as its characterization through the immunoassays and bioassays. A bacterial protein expression vector system based on the phage T7 promoter and histidine tag was used for the expression and purification. The recombinant single chain beta-alpha equine chorionic gonadotropin (reCG) encoding gene was constructed with beta and alpha sequences according to its biologically active counterpart. It was successfully cloned and when expressed in E.coli BL21C host, the purified recombinant protein was found to be active as revealed by enzyme linked immunosorbent assay (ELISA) and Western blotting. However, it was not found to exhibit any significant activity in vivo when tested in the mice |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Animal Research |
| NAAS Rating: | 6.4 |
| Volume No.: | Not Available |
| Page Number: | Not Available |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | DOI: 10.18805/ijar.B-3571 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13941 |
| Appears in Collections: | AS-NRCE-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| IJAR-2018.pdf | 403.28 kB | Adobe PDF | View/Open |
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