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http://krishi.icar.gov.in/jspui/handle/123456789/13976| Title: | Selection, characterization and potency determination of a Bacillus thuringiensis kurstaki isolate toxic to major lepidopteran pests |
| Other Titles: | Not Available |
| Authors: | P. S. Vimala Devi V. Vineela |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR-Indian Institute of Oilseeds Research |
| Published/ Complete Date: | 2016-01-01 |
| Project Code: | AMAAS 1009764 |
| Keywords: | Bacillus thuriungiensis potency Spodoptera litura Helicoverpa armigera Plutella xylostella Achaea janata |
| Publisher: | Connect journals |
| Citation: | Vimala Devi, P.S. and Vineela, V. 2016. Selection, characterization and potency determination of a Bacillus thuringiensis kurstaki isolate toxic to major lepidopteran pests. Biopesticides International, 12(2), 139-148 |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Screening 50 isolates of Bacillus thuringiensis (Bt) kurstaki from soil samples of Rajasthan and Telangana states, India led to identification of 10 isolates promising against the polyphagous pests Spodoptera litura (Fabricius) and Helicoverpa armigera (Hübner). rep-PCR revealed distinctness of one isolate DOR Bt-127 with cry1Ac, 1Ae, 1C, 1E, 2Aa, 2Ab genes coding for toxins effective against a wide range of insect pests. Spore-crystal mixture of Bt-127 had a high spore count of 9x1022/g and delta endotoxin content 18.28%, possessed bipyramidal crystals (0.64-2.12 µm) and ellipsoidal spores (1.92-2.2 µm). SDS-PAGE resulted in a 62 kDa band corresponding to the Cry1 toxin. Potencies against the less susceptible pests S. litura and S. exigua (Hübner) were 71309 and 46205 SU/mg respectively while potencies against the susceptible pests H. armigera, Plutella xylostella (Linnaeus) and Achaea janata (Linnaeus) were 35844, 26571 and 51667 IU/mg respectively. DOR Bt-127 holds great promise for management of these important pests overcoming the need for using different Bt varieties. |
| Description: | Not Available |
| ISSN: | ISSN 0973-483X / e-ISSN 0976-9412 |
| Type(s) of content: | Article |
| Sponsors: | AMAAS 1009764. |
| Language: | English |
| Name of Journal: | Biopesticides International |
| NAAS Rating: | 4.76 |
| Volume No.: | 12(2) |
| Page Number: | 139-148 |
| Name of the Division/Regional Station: | Crop Protection |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/13976 |
| Appears in Collections: | CS-IIOR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| vimal devi and Vineela, 2016.pdf | 187.87 kB | Adobe PDF | View/Open |
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