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http://krishi.icar.gov.in/jspui/handle/123456789/14495| Title: | Phenotyping and Molecular Marker Analysis for stem-rot Disease Resistance Using F 2 Mapping Population in Groundnut |
| Other Titles: | Not Available |
| Authors: | Snehaben M. Dodia , A.L. Rathnakumar Gyan P. Mishra , Radhakrishnan T. Binal Josh ,P.P. Thirumalaisamy ,,Narendra Kumar,Sumitra Chanda,Sumitra Chanda,Abhay Kumar,and Chandramohan Sangh |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Directorate of Groundnut Research |
| Published/ Complete Date: | 2016-01-01 |
| Project Code: | Not Available |
| Keywords: | Arachishypogaea ,Sclerotiumrolfsii , SSR markers, Bulkedsegregant analysis |
| Publisher: | Serials Publication |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Sclerotium rolfsii gene/QTLs can facilitate the identification of resistant genotypes. In the present study, a stem-rot susceptible genotype population was used for SSR marker analysis. For the phenotypic data, F (TG-37A) and a stem-rot resistant genotype (NRCG CS85) were crossed and their F 2:3 2 progenies were screened for stem-rot disease incidence. Parental polymorphism survey was done using 1266 SSR primer pairs so as to identify the polymorphic markers. Among these SSRs, 52 were found to be polymorphic between the parental combination (TG-37A x NRCG CS85).These markers were further utilized for bulked segregant analysis (BSA). Among the polymorphic SSRs, three primers DGR294, DGR470 and DGR510 were able to distinguish both resistant and susceptible bulks and individual plants constituting the bulks. Further genotyping of whole population using identified markers is under way, which may confirm the linkage of putatively linked markers to with the stem-rot resistance. |
| Description: | Not Available |
| ISSN: | ISSN: 0254-8755 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | International Journal of Tropical Agriculture |
| NAAS Rating: | 3.94 |
| Volume No.: | 34(4) |
| Page Number: | 1135-1139 |
| Name of the Division/Regional Station: | Crop Improvement |
| Source, DOI or any other URL: | Not Available |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/14495 |
| Appears in Collections: | CS-DGR-Publication |
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