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http://krishi.icar.gov.in/jspui/handle/123456789/17380
Title: | Transformation of Tomato with cry2AX1 gene of Bacillus thuringiensis |
Other Titles: | Not Available |
Authors: | Ruturaj Barthake R., A. Naveenkumar, P. Nandeesha, R. Manikandan, N. Balakrishnan, V. Balasubramani, D. Sudhakar, and V. Udayasuriyan |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Indian Institute of Horticultural Research |
Published/ Complete Date: | 2014-01-01 |
Project Code: | Not Available |
Keywords: | cry2AX1, regeneration, Helicoverpa armigera, tomato transformation |
Publisher: | SERIALS PUBLICATIONS |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Tomato (Solanum lycopersicum L.,) is an important vegetable crop extensively damaged by lepidopteran insect pest, Helicoverpa armigera Hubner (tomato fruit borer). In order to mitigate this problem, an attempt was made to generate transgenic tomato plants resistant to fruit borer. In this study, a regeneration protocol was standardized for a local high yielding genotype, cv. PKM1. Out of nine different combinations of media tested, modified MS medium containing B5 vitamins along with zeatin exhibited higher regeneration efficiency. Cotyledonary explants of cv. PKM1 were transformed with an indigenous synthetic cry2AX1 gene encoding insecticidal crystal protein of Bacillus thuringiensis through Agrobacterium mediated transformation. Screening by PCR revealed presence of cry2AX1 gene in five out of nine putative transformants; two of the five PCR positive transformants showed expression of Cry2AX1 protein in qualitative ELISA. The two ELISA positive plants showed low level of mortality in H. armigera and significant reduction in feeding of leaf area, in detached leaf bioassay. Selection of cry2AX1 transgenic tomato plants by quantitative ELISA for high level of gene expression will be useful for getting plants with desirable level of insect protection. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Research Paper |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | International Journal of Tropical Agriculture |
NAAS Rating: | 3.94 |
Volume No.: | 32 |
Page Number: | 3-4 |
Name of the Division/Regional Station: | Biotechnology |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/17380 |
Appears in Collections: | HS-IIHR-Publication |
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