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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/17618
Title: | Rapid and sensitive detection of Xanthomonas axonopodis pv. punicae infecting pomegranate using loop-mediated isothermal amplification. |
Other Titles: | Not Available |
Authors: | T. R. Usharani, S. Meenakshi, C. Sunisha, H. D. Sowmya,. Shivshankar G Kumbdale, S. Poovarasan and S. Mohandas |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Indian Institute of Horticultural Research |
Published/ Complete Date: | 2017-04-13 |
Project Code: | HORTIIHRCIL2015:110(7.1) |
Keywords: | pomegranate LAMP Xanthomonas |
Publisher: | NATIONAL ENVIRONMENTALISTS ASSOCIATION, INDIA. |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | In the present study, Loop – mediated isothermal amplification (LAMP) assay was used as a novel tool fordiagnosis of Xanthomonas axonopodis pv. punicae (Xap), a gram negative host specific bacterium causingbacterial blight of pomegranate (Punica granatum). LAMP assay based on the amplification of the Gyrase â (gyrâ)gene showed the detection limit of 1fg/μl of purified Xap DNA when compared with conventional PCR showingamplification only upto 1pg/μl concentration of DNA. The time limit of detection was found to be 45 and 60 minof incubation. The sensitivity of this LAMP assay was determined to be 10 copies per reaction mixture as againstsingle copy detection using real time PCR.The LAMP assay was able to detect Xap from crude extract of theinfected samples collected from different locations without isolating the pathogen or purified genomic DNA.Pathogenic extract from infected leaf was visualized as increase in turbidity as well as color change from violet tosky blue by pre addition of HNB. LAMP assay showed a great potential for monitoring the disease incidence andit could prove to be a powerful supplemental tool for current diagnostic methods |
Description: | Not Available |
ISSN: | 0973-7049 |
Type(s) of content: | Article |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | The Bioscan |
Volume No.: | 12(2) |
Page Number: | 887-892 |
Name of the Division/Regional Station: | Division of Biotechnology |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/17618 |
Appears in Collections: | HS-IIHR-Publication |
Files in This Item:
File | Description | Size | Format | |
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RApid detection LAMP.pdf | 609.01 kB | Adobe PDF | View/Open |
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