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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/18120
Title: | Purification and characterization of prophenoloxidase from cotton bollworm, Helicoverpa armigera |
Other Titles: | Not Available |
Authors: | Hanumanth G. Goudru Sathish Kumar Senigala K. Jayalakshmi Chandish R. Ballal Hari C. Sharma Kuruba Sreeramulu |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | Department of Biochemistry, Gulbarga University Molecular Biophysics Unit, Indian Institute of Science, Bangalore Agricultural Research Station, University of Agricultural Sciences ICAR::National Bureau of Agricultural Insect Resources International Crops Research Institute for Semi Arid Tropics (ICRISAT), Andhra pradesh Department of Biochemistry, Gulbarga University |
Published/ Complete Date: | 2012-11-28 |
Project Code: | Not Available |
Keywords: | Not Available |
Publisher: | Wiley |
Citation: | Not Available |
Series/Report no.: | Not Available; |
Abstract/Description: | Phenoloxidases are oxidative enzymes, which play an important role in both cell mediated and humoral immunity. Purification and biochemical characterization of prophenoloxidase from cotton bollworm, Helicoverpa armigera (Hübner) were carried out to study its biochemical properties. Prophenoloxidase consists of a single polypeptide chain with a relative molecular weight of 85 kDa as determined by SDS–PAGE, MALDI–TOF MS and LC–ESI MS. After the final step, the enzyme showed 71.7 fold of purification with a recovery of 49.2%. Purified prophenoloxidase showed high specific activity and homology with phenoloxidase subunit‐1 of Bombyx mori and the conserved regions of copper binding (B) site of phenoloxidase. Purified prophenoloxidase has pH optima of 6.8 and has high catalytic efficiency towards the dopamine as a substrate in comparison to catechol and L‐Dopa. The PO activity was strongly inhibited by phenylthiourea, thiourea, dithiothreitol and kojic acid. |
Description: | Not Available |
ISSN: | Not Available |
Type(s) of content: | Journal |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Entomological Research |
NAAS Rating: | 6.81 |
Volume No.: | 43(1) |
Page Number: | 55-62 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | https://doi.org/10.1111/1748-5967.12002 |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/18120 |
Appears in Collections: | CS-NBAIR-Publication |
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