Molecular screening of rice genotypes using linked markers for gall midge resistance gene, Gm4

Abstract

Forty-eight rice genotypes comprising eighteen donors and thirty high yielding varieties were evaluated against biotype 2 of gall midge under glass house conditions at seedling stage. Three genotypes exhibited immune reaction while seven genotypes showed the high level of resistance to gall midge infestation. Two genotypes were moderately resistant while two genotypes were moderately susceptible. Thirtyfour genotypes showed the susceptible reaction. These 48 rice genotypes were amplified with Gm4 resistance gene linked four markers RM22550, RM22551, RM547 and RM22555. Based on the amplification pattern, the resistance gene Gm4 from the cultivar PTB10 or Abhaya can be pyramided with other gall midge resistance genes present in the seven donors, Kavya, Samridhi, Phalguna, Dukong I, Suraksha, Aganni and BG380-2 in the background of twenty susceptible high-yielding varieties, Swarna, Samba Mashuri, Daya, Gajapati, Surendra, Ramachandi, CRdhan 500, Pooja, Tapaswini, Satabdi, Kharabela, B- 95-1, CRdhan 300, Kanchan, Durga, Reeta, Naveen, Mahanadi, Ranjit and Sarathi through MAS breeding programs for development of durable gall midge resistance against biotypes 1, 2, 3, 4, 5, 6 and 7.