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http://krishi.icar.gov.in/jspui/handle/123456789/19561| Title: | Purification and characterization of a novel thermostable antifungal protein with chitinase activity from mung bean |
| Other Titles: | Not Available |
| Authors: | D.S. Solanki S. Kumar K. Parihar K. Sharma P. Gehlot S.K. Singh R. Pathak |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | Jai Narain Vyas University, Jodhpur ICAR::Central Arid Zone Research Institute |
| Published/ Complete Date: | 2018-01-01 |
| Project Code: | Not Available |
| Keywords: | Antifungal protein Chitinase activity Chromatography SDS-PAGE Thermostability |
| Publisher: | Not Available |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Antifungal proteins are produced by many plant species and participate in defence mechanisms against number of fungal pathogens. The main objective of the study was to purify and characterize the thermo-stable antifungal compound with chitinase activity from mung bean seeds to assess their antifungal potency. The antifungal protein was isolated through ammonium sulphate precipitation method followed by its purification through ion-exchange chromatography. The purified protein was characterized by evaluating its antifungal efficacy, thermal stability, chitinase activity andSDSPAGEprofiling. Molecular mass of characterized antifungal protein was 50.6 kDa. Purified protein exhibited antifungal activity against pathogenic fungi and and sustained its thermo-stability up to 60°C with chitinase activity. The isolated antifungal proteins showed unique column chromatographic behaviour, molecular weight, specificity of chitinase activity and relatively high thermo-stability with potent antifungal activity. It can be used in different biomedical and pharmaceutical applications as bio-pesticides. Vigna radiata Macrophomina phaseolina Magnaporthe grisea Characterization of antifungal protein |
| Description: | Not Available |
| ISSN: | Not Available |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Journal of Environmental Biology |
| NAAS Rating: | 6.78 |
| Volume No.: | 39 |
| Page Number: | 406-412 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | http://doi.org/10.22438/jeb/39/3/MRN-623 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/19561 |
| Appears in Collections: | NRM-CAZRI-Publication |
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