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http://krishi.icar.gov.in/jspui/handle/123456789/19847| Title: | Assessment of fluorescence polarization assay: a candid diagnostic tool in Brucella abortus strain 19 vaccinated areas. |
| Other Titles: | Not Available |
| Authors: | Kalleshamurthy T Shekar R Niranjanamurthy HH Kritiga N Shome BR Bambal RG Sairiwal L Barbuddhe SB Sahare A Kilari S Rahman H Shome R |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::National Institute of Veterinary Epidemiology and Disease Informatics Jain University, Centre for Post Graduate Studies, Jaya Nagar , Bengaluru- 560041, India Department of Animal Husbandry, Dairying & Fisheries, Krishi Bhavan, New Delhi 110001, India ICAR::National Research Centre on Meat Intervet India Pvt. Ltd. MSD Animal Health, Briahnagar, Off Pune-Nagar Road, Wagholi - 412 207 Pune, India International Livestock Research Institute (ILRI), NASC Complex, CG Centre, , Pusa, New Delhi -110 012 |
| Published/ Complete Date: | 2018-10-22 |
| Project Code: | Not Available |
| Keywords: | ELISA Rose Bengal plate test S19 vaccination fluorescence polarization assay |
| Publisher: | Richmond : Wiley-Blackwell publisher |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Brucellosis caused by the bacteria of the genus Brucella is an important zoonosis and constitutes a serious public health hazard. Brucellosis is diagnosed mainly by the Rose Bengal plate test and indirect ELISA, both of which have poor specificity because false positive serological reactions occur when screening animals that have been vaccinated with B. abortus S19. Fluorescence polarization assay (FPA) was evaluated for screening samples from cattle and buffalo calves with persistent antibody titers induced by vaccination. The standardized FPA exhibited relative sensitivity and specificity of 0.94 and 0.95, respectively, and the area under the curve, kappa and accuracy were 0.98, 0.87 and 0.95, respectively. Comparison of competitive ELISA and FPA revealed that, FPA is more specific than competitive ELISA. The high specificity, sensitivity and 95% accuracy of FPA indicate that, it is suitable for testing vaccinated animals because it can distinguish between infected from vaccinated animals. |
| Description: | Not Available |
| ISSN: | 3035-0322 |
| Type(s) of content: | Research Paper |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Microbiology and Immunology |
| NAAS Rating: | 7.57 |
| Volume No.: | 62(11) |
| Page Number: | 694-701 |
| Name of the Division/Regional Station: | Animal science |
| Source, DOI or any other URL: | https://doi.org/10.1111/1348-0421.12654 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/19847 |
| Appears in Collections: | AS-NIVEDI-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| kalleshamurthy2018.pdf | 637.97 kB | Adobe PDF | View/Open |
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