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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/23525
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Shanmugam M, Mahapatra, R.K. | en_US |
dc.date.accessioned | 2019-10-17T10:05:26Z | - |
dc.date.available | 2019-10-17T10:05:26Z | - |
dc.date.issued | 2019-08-03 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | 1678-4324 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/23525 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The present study evaluated the effect of cryoprotectants, semen diluents and chicken lines during pellet method of semen cryopreservation. Three different experiments were conducted; Experiment 1 - semen was cryopreserved using dimethylformamide (DMF) at 6% and 9% concentrations in two semen diluents (Lake and Ravie diluent and TES/NaCl diluent), Experiment 2 - semen was cryopreserved using dimethylacetamide (DMA) at 6% and 9% with or without sucrose (100mM), Experiment 3- semen from two chicken lines (PD1 and PD6) was cryopreserved using DMA (6% and 9%). Semen was evaluated pre and post cryopreservation for progressive motility, live and abnormal sperm. Semen pellets were stored in cryovials for at least seven days before examination and insemination. Thawed semen was inseminated intravaginaly to study fertility. All the parameters studied were significantly lower (p<0.05) in cryopreserved semen. DMF in Lake and Ravie diluent gave very low fertility and TES/NaCl diluent no fertile eggs. DMA as cryoprotectant gave fertility up to 9.22 %. Addition of sucrose along with DMA produced fertility similar to other cryopreservation treatment groups. No difference in in vitro semen parameters between chicken lines was observed. There is difference in cryopreservation outcome due to semen diluent and type of cryoprotectant. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Instituto de Tecnologia do Paraná - Tecpar, Brazil | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | chicken; diluent; fertility; pellet cryopreservation; semen | en_US |
dc.title | Pellet Method of Semen Cryopreservation: Effect of Cryoprotectants, Semen Diluents and Chicken Lines | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | ANSCDPRSIL201500900058 | en_US |
dc.publication.journalname | Brazilian Archives of Biology and Technology | en_US |
dc.publication.volumeno | 62 | en_US |
dc.publication.pagenumber | e19180188 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | http://dx.doi.org/10.1590/1678-4324-2019180188 | en_US |
dc.publication.authorAffiliation | ICAR::Directorate of Poultry Research | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
dc.publication.naasrating | 6.58 | en_US |
Appears in Collections: | AS-DPR-Publication |
Files in This Item:
File | Description | Size | Format | |
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Pellet method semen cryopreseration-babt.pdf | 233.03 kB | Adobe PDF | View/Open |
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