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http://krishi.icar.gov.in/jspui/handle/123456789/23527| Title: | Effect of different cryoprotectants on post-thaw semen parameters and fertility in Nicobari chicken |
| Other Titles: | Not Available |
| Authors: | Shanmugam M, Pranay Kumar, K., Mahapatra, R.K., Anand Laxmi, N. |
| ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
| Author's Affiliated institute: | ICAR::Directorate of Poultry Research |
| Published/ Complete Date: | 2018-04-01 |
| Project Code: | ANSCDPRSIL201800100067 |
| Keywords: | Chicken, Cryopreservation, Fertility, Semen |
| Publisher: | Indian Poultry Science Association |
| Citation: | Not Available |
| Series/Report no.: | Not Available; |
| Abstract/Description: | Three different cryoprotectants were evaluated for Nicobari chicken semen cryopreservation. Semen mixed with any one of the cryoprotectants, 12% methylacetamide (MA), 9% dimethylacetamide (DMA) or 4% dimethylsulfoxide (DMSO) was cryopreserved in 0.5ml French straws by exposing to nitrogen vapours for 30 min followed by plunging into liquid nitrogen. The semen straws were thawed at 5°C for 100 sec in ice water for in vitro evaluation and insemination. Post-thaw semen samples were assessed for different in vitro semen parameters and fertility. Post-thaw sperm motility, live sperm, MTT dye reduction test and fertility were significantly (P<0.05) lower in cryopreservation treatments. The abnormal sperm percent was significantly (P<0.05) higher in all cryopreservation treatments. The seminal plasma lipid peroxidation was significantly (P<0.05) higher in 9% DMA and 4% DMSO treatments. No fertile egg was obtained in 12% MA and 9% DMA treatments. In conclusion, the three cryoprotectants at the tested concentrations are not useful for cryopreserving Nicobari chicken semen. |
| Description: | Not Available |
| ISSN: | 0019-5529 |
| Type(s) of content: | Article |
| Sponsors: | Not Available |
| Language: | English |
| Name of Journal: | Indian Journal of Poultry Science |
| NAAS Rating: | 5.85 |
| Volume No.: | 53 |
| Page Number: | 208-211 |
| Name of the Division/Regional Station: | Not Available |
| Source, DOI or any other URL: | 10.5958/0974-8180.2018.00040.5 |
| URI: | http://krishi.icar.gov.in/jspui/handle/123456789/23527 |
| Appears in Collections: | AS-DPR-Publication |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| Different cryoprotectants in Nicobari semen cryopreservation.pdf | 48.66 kB | Adobe PDF | View/Open |
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