Ellagic and ferulic acids protect arsenic-induced male reproductive toxicity via regulating Nfe2l2, Ppargc1a and StAR expressions in testis

Abstract

Arsenic (As) - induced oxidative stress causes male reproductive toxicity apart from its other generalized systemic effects. Some phytochemicals through their antioxidant properties might help to overcome such toxic effects. The aim of the study was to elucidate the protective role of the selected phytochemicals, ellagic and ferulic acids against the As-induced reproductive toxicity. Forty two healthy male Swiss albino mice were randomly assigned to six groups (each @ n = 7). Group A served as the control, while group B received 200 ppm of As through drinking water. The group C and D mice were administered Per os (P.O) with 50 mg/kg BW of ellagic and ferulic acids, respectively on alternate days. Group E or F received 50 mg of ellagic or ferulic acid + 200 ppm of As for forty days. Ellagic and/ ferulic acid significantly reduced the accumulation of As, protein carbonylation (PC), lipid peroxidation (LPO) in addition to altering the antioxidant enzymes (CAT and SOD) activities, reduced glutathione (GSH) and total antioxidant capacity (TAC) in the testicular tissues. A significantly (p < 0.05) altered sperm functions (viability, functional membrane integrity, Δψm and sperm kinematics like total motility, rapid, progressive motile) and type-A (STR > 80%, ALH > 2.5 μm) and testicular damage induced by the As were ameliorated (p < 0.05) by the phytochemical treatments. These phytochemicals due to their antioxidant activities were found to attenuate the As-induced oxidative stress, testicular damage, and sperm abnormalities via regulating the expressions of Nfe2l2, StAR and Ppargc1a. The study revealed that ellagic and ferulic acids might be potential therapeutic options to protect the male reproductive system from As-poisoning.