KRISHI
ICAR RESEARCH DATA REPOSITORY FOR KNOWLEDGE MANAGEMENT
(An Institutional Publication and Data Inventory Repository)
"Not Available": Please do not remove the default option "Not Available" for the fields where metadata information is not available
"1001-01-01": Date not available or not applicable for filling metadata infromation
"1001-01-01": Date not available or not applicable for filling metadata infromation
Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/25330
Title: | Incidence of Aspergillus fungal species in cured fish of Gujarat based on internal transcribed spacer regions 1 and 4 |
Other Titles: | Not Available |
Authors: | Sivaraman, G.K. Vishnuvinayagam, S. Jha, A.K. Remya, S. Renuka, V. Ajeesh, K. Vanik, D. Prasad, M.M. |
ICAR Data Use Licennce: | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf |
Author's Affiliated institute: | ICAR::Central Institute of Fisheries Technology Mumbai Research Centre of Central Institute of Fisheries Technology Veraval Research Centre of ICAR-Central Institute of Fisheries Technology, Veraval |
Published/ Complete Date: | 2017-11-21 |
Project Code: | Not Available |
Keywords: | Not Available |
Publisher: | ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch |
Citation: | Sivaraman, G.K.,Vishnuvinayagam, S.,Jha, A.K.,Remya, S.,Renuka, V.,Ajeesh, K.,Vanik, D. and Prasad, M.M. (2017) Incidence of Aspergillus fungal species in cured fish of Gujarat based on internal transcribed spacer regions 1 and 4. In: (Thomas, S.N., Rao, B.M., Madhu, V.R., Asha, K.K., Binsi, P.K., Viji, P., Sajesh, V.K. and Jha, P.N., Eds.) Fostering Innovations in Fisheries and Aquaculture: Focus on Sustainability and Safety – Book of Abstracts, 11th Indian Fisheries and Aquaculture Forum, ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch, 21-24 November, 2017, Kochi, India, pp. 390-391. |
Series/Report no.: | Not Available; |
Abstract/Description: | Fungal infestations cause quality losses and often pose serious health hazards to the consumers. The sun dried fish samples such as ribbon fish, bombay duck, catfish, horse mackerel, dhoma, leather jacket, prawn and acetes were collected from the dried fish processing facilities and commercial markets from Veraval and Nawabhandar in Gujarat. Ten gram of the fish samples were homogenised in sterile normal saline and serial dilution was made from 10-1 to 10-6 and spread onto a duplicate plate of Rose Bengal Chloramphenicol Agar and incubated at 25 to 30 degree celsius for 5 days. The total fungal counts ranged from 2.0 x 101 to 5.0 x 102 cfu. per gram and the higher counts were frequently seen in the ribbon fish sample. The morphologically distinct colony was streaked on to Sabouraud Dextrose Agar for DNA isolation. The fungal DNA was isolated and purified as per the manufacturer’s guidelines and PCR was carried out with the universal primers for the 18 S gene with the ITS1 and ITS4 regions of 670 bp. The DNA sequences were submitted to the European Nucletide Archives and found that Aspergillus nidulans, Aspergillus oryzae, Aspergillus flavus, Aspergillus sydowii, A. foetidus and Aspergillus rugulosa. The highest similarity was observed between Aspergillus flavus with Aspergillus oryzae and Aspergillus nidulans with Aspergillus rugulosa. The least genetic similarity was found between Aspergillus flavus with Aspergillus rugulosa and Aspergillus oryzae with Aspergillus rugulosa. The phylogenetic tree revealed that two clade were formed among the fungal species with one clade belongs to Aspergillus flavus, Aspergillus rugulosa and Aspergillus sydowii and the another with Aspergillus flavus and Aspergillus oryzae. The present study shows the presence of different pathogenic fungal species which can be from different sources of contamination at various stages of dry fish processing. |
Description: | Not Available |
ISBN: | 978-81-933623-1-0 |
Type(s) of content: | Other |
Sponsors: | Not Available |
Language: | English |
Name of Journal: | Not Available |
Volume No.: | Not Available |
Page Number: | 390-391 |
Name of the Division/Regional Station: | Not Available |
Source, DOI or any other URL: | Not Available |
URI: | http://krishi.icar.gov.in/jspui/handle/123456789/25330 |
Appears in Collections: | FS-CIFT-Publication |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
390_Incidence of Aspergillus.pdf | 186.78 kB | Adobe PDF | View/Open |
Items in KRISHI are protected by copyright, with all rights reserved, unless otherwise indicated.