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Please use this identifier to cite or link to this item: http://krishi.icar.gov.in/jspui/handle/123456789/25346
Title: Characterization of Vibrio parahaemolyticus strains isolated from marine fish and shell fish
Other Titles: Not Available
Authors: Rao, B.M.
Joseph, T.C.
Lalitha, K.V.
ICAR Data Use Licennce: http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf
Author's Affiliated institute: Visakhapatnam Research Centre of ICAR-Central Institute of Fisheries Technology
ICAR- Central Institute of Fisheries Technology, Cochin, India
Published/ Complete Date: 2017-11-21
Project Code: Not Available
Keywords: Not Available
Publisher: ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch
Citation: Rao, B.M.,Joseph, T.C. and Lalitha, K.V. (2017) Characterization of Vibrio parahaemolyticus strains isolated from marine fish and shell fish. In: (Thomas, S.N., Rao, B.M., Madhu, V.R., Asha, K.K., Binsi, P.K., Viji, P., Sajesh, V.K. and Jha, P.N., Eds.) Fostering Innovations in Fisheries and Aquaculture: Focus on Sustainability and Safety – Book of Abstracts, 11th Indian Fisheries and Aquaculture Forum, ICAR-Central Institute of Fisheries Technology, Kochi and Asian Fisheries Society, Indian Branch, 21-24 November, 2017, Kochi, India, pp. 402-403.
Series/Report no.: Not Available;
Abstract/Description: A total of forty two samples comprising of marine fish and shellfish samples comprising of farmed Litopenaeus vannamei, farmed Litopenaeus vannamei-cooked, farmed Penaeus monodon , and marine shrimp procured from Visakhapatnam, Andhra Pradesh were analysed for the presence of V. parahaemolyticus. Nine samples of marine fish and one sample of marine shrimp were positive for the presence of V. parahaemolyticus but all the farmed shrimp samples were negative. The V. parahaemolyticus isolates were confirmed using V. parahaemolyticus specific PCR targeting tl and flaE genes. All the V. parahaemolyticus isolates were negative for thermostable direct haemolysin (tdh) gene and none of them belonged to the highly virulent O3 colon K6 serogroup. The genetic heterogeneity of V. parahemolyticus isolates from marine fish was tested by employing ERIC- PCR. V parahaemolyticus isolates were clustered in to four distinct groups at 55 per cent similarity level. Group one comprised of the MTCC and ATCC type cultures and a field isolate of V. parahaemolyticus. However, majority of the V. parahaemolyticus isolates from marine fish were clustered in Group 2 at 80 per cent similarity level. The V. parahaemolyticus isolates VP4 and VP8 showed lesser similarity of 68 per cent and 55 per cent respectively and were delineated separately. Thyme oil at 0.25 per cent completely inhibited caseinase activity of V. parahaemolyticus whereas gelatinase activity was inhibited at 0.5 per cent level. Amylase and DNAse activities of the V. parahaemolyticu isolates decreased with increasing concentration of thyme oil but enzymatic activity was still observed at 1 per cent thyme oil level.
Description: Not Available
ISBN: 978-81-933623-1-0
Type(s) of content: Other
Sponsors: Not Available
Language: English
Name of Journal: Not Available
Volume No.: Not Available
Page Number: 402-403
Name of the Division/Regional Station: Not Available
Source, DOI or any other URL: Not Available
URI: http://krishi.icar.gov.in/jspui/handle/123456789/25346
Appears in Collections:FS-CIFT-Publication

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