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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/26683
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DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ananya Mukherjee, Randhir Singh, Sreeram Udayan, Sayan Biswas, Purushotham Pothula, Saumya Manmadhan, Geen George, Shilpa Dilip Kumar, Ranabir Das, Balaji M Rao, Akash Gulyani | en_US |
dc.date.accessioned | 2019-12-03T10:12:12Z | - |
dc.date.available | 2019-12-03T10:12:12Z | - |
dc.date.issued | 2019-01-01 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | Not Available | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/26683 | - |
dc.description | Not Available | en_US |
dc.description.abstract | Cell behavior is controlled through spatio-temporally localized protein activity. Despite unique and often contradictory roles played by Src-family-kinases (SFKs) in regulating cell physiology, activity patterns of individual SFKs have remained elusive. Here, we report a biosensor for specifically visualizing active conformation of SFK-Fyn in live cells. We deployed combinatorial library screening to isolate a binding-protein (F29) targeting activated Fyn. Nuclear-magnetic-resonance (NMR) analysis provides the structural basis of F29 specificity for Fyn over homologous SFKs. Using F29, we engineered a sensitive, minimally-perturbing fluorescence-resonance-energy-transfer (FRET) biosensor (FynSensor) that reveals cellular Fyn activity to be spatially localized, pulsatile and sensitive to adhesion/integrin signaling. Strikingly, growth factor stimulation further enhanced Fyn activity in pre-activated intracellular zones. However, inhibition of focal-adhesion-kinase activity not only attenuates Fyn activity, but abolishes growth-factor modulation. FynSensor imaging uncovers spatially-organized, sensitized signaling clusters, direct crosstalk between integrin and growth-factor-signaling, and clarifies how compartmentalized Src-kinase activity may drive cell fate. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | Cold Spring Harbor Laboratory | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Not Available | en_US |
dc.title | A Fyn-specific biosensor reveals localized, pulsatile kinase activity and spatially regulated signaling crosstalk | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Research Paper | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | bioRxiv | en_US |
dc.publication.volumeno | Not Available | en_US |
dc.publication.pagenumber | Pages 726133 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | https://doi.org/10.1101/726133 | en_US |
dc.publication.sourceUrl | https://scholar.google.com/scholar?oi=bibs&cluster=9567993737596545384&btnI=1&hl=en | en_US |
dc.publication.authorAffiliation | ICAR::Indian Institute of Soil Science | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | NRM-IISS-Publication |
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