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Please use this identifier to cite or link to this item:
http://krishi.icar.gov.in/jspui/handle/123456789/26879
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hussain M, Chakravarty P., Soren S., Deori S., Begum S. S | en_US |
dc.date.accessioned | 2019-12-04T05:50:03Z | - |
dc.date.available | 2019-12-04T05:50:03Z | - |
dc.date.issued | 2015-02-21 | - |
dc.identifier.citation | Not Available | en_US |
dc.identifier.issn | 0976-0555 | - |
dc.identifier.uri | http://krishi.icar.gov.in/jspui/handle/123456789/26879 | - |
dc.description | Not Available | en_US |
dc.description.abstract | The study was undertaken to see the effect of recovery techniques and culture media on in-vitro maturation (IVM) of oocytes indigenous cattle of Assam. Oocytes were collected by aspiration and dissection techniques and were graded as A, B, C and D on the basis of their cumulus cell layers surrounding the zona pellucida. Oocytes with A, B and C categories were subjected to IVM using three different media (TCM-199, Ham's F-12 and Ham's F-10) with same additives (Sodium pyruvate, Gentamicin, L-glutamine, Porcine FSH, Follicular Fluid and HIOCS). Percentage of oocytes with expansion of cumulus cells was found to be the highest in dissection technique using TCM-199 media in comparison to Ham's F-12 and Ham's F-10 using same additives. However, it did not differ significantly between techniques. Incidence of Metaphase-I + Metaphase-II was the highest in oocytes recovered by dissection technique and cultured in TCM-199 with additives when incubated at 38.5°C for 24 hours and it differed significantly between media but there was no significant difference between the recovery techniques. In conclusion, the culture medium TCM-199 along with additives may give better IVM rate of the oocytes recovered by dissection technique from indigenous cattle ovaries of Assam. | en_US |
dc.description.sponsorship | Not Available | en_US |
dc.language.iso | English | en_US |
dc.publisher | ARCC JOURNALS | en_US |
dc.relation.ispartofseries | Not Available; | - |
dc.subject | Cattle oocytes, Culture media, In-vitro maturation, Recovery technique | en_US |
dc.title | Effect of recovery technique and culture media on in vitro maturation of indigenous cattle oocytes of Assam | en_US |
dc.title.alternative | Not Available | en_US |
dc.type | Article | en_US |
dc.publication.projectcode | Not Available | en_US |
dc.publication.journalname | Indian Journal of Animal Research | en_US |
dc.publication.volumeno | 49 | en_US |
dc.publication.pagenumber | 51-54 | en_US |
dc.publication.divisionUnit | Not Available | en_US |
dc.publication.sourceUrl | 10.5958/0976-0555.2015.00011.4 | en_US |
dc.publication.authorAffiliation | ICAR::National Research Centre on Pig | en_US |
dc.ICARdataUseLicence | http://krishi.icar.gov.in/PDF/ICAR_Data_Use_Licence.pdf | en_US |
Appears in Collections: | AS-NRCP-Publication |
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